Rabbit anti-Human, Mouse PDIA6 Polyclonal Antibody | anti-PDIA6 antibody

PDIA6, ID (K159) (Protein Disulfide-isomerase A6, Protein Disulfide Isomerase P5, Thioredoxin Domain-containing Protein 7, Endoplasmic Reticulum Protein 5, ER Protein 5, ERp5, TXNDC7, PDIA6) (Biotin)

Cat. Num. AAA6492482

• Gene Names: PDIA6; P5; ERP5; TXNDC7
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PDIA6; Polyclonal Antibody; ID (K159) (Protein Disulfide-isomerase A6; Protein Disulfide Isomerase P5; Thioredoxin Domain-containing Protein 7; Endoplasmic Reticulum Protein 5; ER Protein 5; ERp5; TXNDC7; PDIA6) (Biotin); anti-PDIA6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDIA6. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PDIA6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PDIA6 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PDIA6 antibody (Center K159) in Y79 cell line lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PDIA6 antibody (Center K159) in mouse stomach tissue lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using PDIA6 Antibody (Center K159)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PDIA6 antibody

Antibodies; Enzymes; Isomerase

References

1. Hayano, T., Gene 164(2), 377-378 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 5031973
• NCBI GeneID: 10130
• NCBI Accession #: NP_005733
• NCBI GenBank Nucleotide #: NM_005742.3
• UniProt Accession #: Q15084; Q53RC7; Q6ZSH5; Q99778; B3KY95; B5MCQ5; B7Z254; B7Z4M8; F8WA83
• Molecular Weight: 48.5kDa (442aa) confirmed by MALDI-TOF
• NCBI Official Full Name: protein disulfide-isomerase A6 isoform d
• NCBI Official Synonym Full Names: protein disulfide isomerase family A member 6
• NCBI Official Symbol: PDIA6
• NCBI Official Synonym Symbols: P5; ERP5; TXNDC7
• NCBI Protein Information: protein disulfide-isomerase A6
• UniProt Protein Name: Protein disulfide-isomerase A6
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA6
• UniProt Synonym Gene Names: ERP5; P5; TXNDC7; ER protein 5; ERp5
• UniProt Entry Name: PDIA6_HUMAN

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]

Uniprot Description

PDIA6: May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.3.4.1; Isomerase

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; plasma membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; unfolded protein response; cell redox homeostasis; apoptotic cell clearance