Rabbit anti-Human F2RL2 Polyclonal Antibody | anti-F2RL2 antibody

F2RL2, NT (F2RL2, PAR3, Proteinase-activated receptor 3, Coagulation factor II receptor-like 2, Thrombin receptor-like 2) (APC)

Cat. Num. AAA6297395

• Gene Names: F2RL2; PAR3; PAR-3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: F2RL2; Polyclonal Antibody; NT (F2RL2; PAR3; Proteinase-activated receptor 3; Coagulation factor II receptor-like 2; Thrombin receptor-like 2) (APC); Thrombin receptor-like 2; anti-F2RL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-F2RL2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: F2RL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-50 amino acids from the N-terminal region of human F2RL2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(F2RL2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the F2RL2 antibody detected the F2RL2 protein (arrow).)

Immunohistochemistry (IHC)

(F2RL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of F2RL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-F2RL2 antibody

Coagulation factor II (thrombin) receptor-like 2 (F2RL2) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL2 is also a member of the protease-activated receptor family and activated by thrombin. F2RL2 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL2 is a cofactor for F2RL3 activation by thrombin. It mediates thrombin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues.

Product Categories/Family for anti-F2RL2 antibody

Antibodies; Coagulation Factors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Popovic, M., et al. J. Thromb. Thrombolysis 30(2):164-171(2010). Van Laer, L., et al. Eur. J. Hum. Genet. 18(6):685-693(2010). Wysoczynski, M., et al. Mol. Cancer Res. 8(5):677-690(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4758326
• NCBI GeneID: 2151
• NCBI Accession #: NP_004092.1
• NCBI GenBank Nucleotide #: NM_004101.3
• UniProt Accession #: O00254; Q52M68; Q7Z3W3; B2R754; B4DQ13
• Molecular Weight: 40,247 Da
• NCBI Official Full Name: proteinase-activated receptor 3 isoform 1
• NCBI Official Synonym Full Names: coagulation factor II thrombin receptor like 2
• NCBI Official Symbol: F2RL2
• NCBI Official Synonym Symbols: PAR3; PAR-3
• NCBI Protein Information: proteinase-activated receptor 3
• UniProt Protein Name: Proteinase-activated receptor 3
• Protein Family: Proteinase-activated receptor
• UniProt Gene Name: F2RL2
• UniProt Synonym Gene Names: PAR3; PAR-3
• UniProt Entry Name: PAR3_HUMAN

NCBI Description

This gene encodes a member of the protease-activated receptor (PAR) family which is a subfamily of the seven transmembrane G protein-coupled cell surface receptor family. The encoded protein acts as a cofactor in the thrombin-mediated cleavage and activation of the protease-activated receptor family member PAR4. The encoded protein plays an essential role in hemostasis and thrombosis. Alternate splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

PAR3: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Interacts with INSC/inscuteable and probably GPSM2. Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: integral to plasma membrane; apical plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; phosphoinositide phospholipase C activity; thrombin receptor activity

Biological Process: platelet activation; metabolic process; response to wounding; blood coagulation