Rabbit anti-Human CBLC Polyclonal Antibody | anti-CBLC antibody

Rabbit Polyclonal to Human CBLC

Cat. Num. AAA240750

• Gene Names: CBLC; CBL-3; RNF57; CBL-SL
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CBLC; Polyclonal Antibody; Rabbit Polyclonal to Human CBLC; Anti-CBLC Antibody (aa444-458) IHC-plus; CBL-3; CBL3; RNF57; SH3-binding protein CBL-C; SH3-binding protein CBL-3; CBL-c; CBL-SL; RING finger protein 57; Human CBLC; anti-CBLC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 444-458 of Human Cbl-c.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1.42 mg/ml (varies by lot)
• Sequence Length: 474

• Applicable Applications for anti-CBLC antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IF, IHC-P (5 ug/ml), IP, WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CBLC.
• Immunogen Type: Synthetic peptide
• Immunogen: CBLC antibody was raised against synthetic peptide from human CBLC.
• Antigen Modification: aa444-458
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CBLC antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-Cbl-c Antibody - Immunoprecipitation and Western Blot. Immunoprecipitation and Western blot of Affinity Purified anti-Cbl-c antibody shows detection of a pre-dominant band at ~52 kD corresponding to Cbl-c (arrowhead) in transfected cell lysates (left panel). Lysates are from Hek 293T cells transfected with empty vector or with Cbl-c. The predicted size of Cbl-c is 52 kD. Size markers in kD are shown to the left of the panel. The right panel shows western blotting after first immunoprecipitating with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.)

Related Product Information for anti-CBLC antibody

CBLC belongs to a family of ubiquitin ligases that are negative regulators of tyrosine kinase signaling downstream of receptor tyrosine kinase and lymphocyte antigen receptors. Monoubiquitin tag promotes sorting of activated receptor and associated proteins into internal vesicles facilitating their lysosomal degradation, while polyubiquitin tag promotes proteasomal degradation. CBLC is expressed in epithelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 195927027
• NCBI GeneID: 23624
• NCBI Accession #: NP_036248.3
• NCBI GenBank Nucleotide #: NM_012116.3
• UniProt Accession #: Q9ULV8; Q8N1E5; Q9Y5Z2; Q9Y5Z3
• Molecular Weight: 47,418 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL-C isoform 1
• NCBI Official Synonym Full Names: Cbl proto-oncogene C, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBLC
• NCBI Official Synonym Symbols: CBL-3; RNF57; CBL-SL
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL-C; RING finger protein 57; SH3-binding protein CBL-3; SH3-binding protein CBL-C; signal transduction protein CBL-C; Cbl proto-oncogene, E3 ubiquitin protein ligase C; Cas-Br-M (murine) ectropic retroviral transforming sequence c; Cas-Br-M (murine) ecotropic retroviral transforming sequence c
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL-C
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CBLC
• UniProt Synonym Gene Names: CBL3; RNF57
• UniProt Entry Name: CBLC_HUMAN

NCBI Description

This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Cbl-c: Regulator of EGFR mediated signal transduction. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleus

Molecular Function: signal transducer activity; zinc ion binding; phosphotyrosine binding; calcium ion binding; SH3 domain binding; ligase activity; epidermal growth factor receptor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor activity; negative regulation of MAP kinase activity; cell surface receptor linked signal transduction; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination