Mouse anti-Mouse, Rat Tau Monoclonal Antibody | anti-tau antibody

Tau, phosphorylated (Ser396)

Cat. Num. AAA609274

• Gene Names: tau; BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Ascites; Ascites
• Synonyms: Tau; Monoclonal Antibody; phosphorylated (Ser396); Anti -Tau; anti-tau antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9B149
• Specificity: Recognizes endogenous levels of Tau at 50-80kD only when phosphorylated at serine 396. Species Crossreactivity
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-tau antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.; Optimal dilution determined by the researcher.
• Immunogen: Human purified Tau (Hoffmann et al., 1997).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from mouse brain and CAD cells, using MBS609274. The phospho-specificity of the antibody was verified by peptide blocking using no peptide, phospho-peptide or nonphospho-peptide.)

Related Product Information for anti-tau antibody

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Product Categories/Family for anti-tau antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21357661
• NCBI GeneID: 326116
• NCBI Accession #: NP_651575.1
• NCBI GenBank Nucleotide #: NM_143318.2
• NCBI Official Full Name: tau
• NCBI Official Symbol: tau
• NCBI Official Synonym Symbols: BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
• NCBI Protein Information: CG31057 gene product from transcript CG31057-RA; tau; CG31057-PA; tau-PA
• Protein Family: Tautomerase