Rabbit anti-Human PCNA Polyclonal Antibody | anti-PCNA antibody

PCNA, CT (Proliferating Cell Nuclear Antigen, Cyclin)

Cat. Num. AAA646028

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; .Purified by immunoaffinity chromatography.
• Synonyms: PCNA; Polyclonal Antibody; CT (Proliferating Cell Nuclear Antigen; Cyclin); Anti -PCNA; anti-PCNA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PCNA. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; .Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 1% BSA, 0.02% thimerosal.

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 2-4ug/ml; Immunohistochemistry (Frozen,Formalin-fixed, paraffin-embedded): 1:100; Immunohistochemistry: Frozen
• Immunogen: Synthetic peptide corresponding to the C-terminal of human PCNA.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with PCNA antibody at 1:100 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PCNA antibody

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

Product Categories/Family for anti-PCNA antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 49456555
• NCBI GeneID: 5111
• UniProt Accession #: Q6FHF5; P12004
• Molecular Weight: 28,706 Da
• NCBI Official Full Name: PCNA
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Protein Information: proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Entry Name: Q6FHF5_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand

By similarity.

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the PCNA family. RuleBase RU003671