PCNA Monoclonal Antibody | anti-PCNA antibody

PCNA Mouse Monoclonal Antibody

Cat. Num. AAA8504224

• Gene Names: PCNA; ATLD2
• Applications: Western Blot, Immunohistochemistry
• Synonyms: PCNA; Monoclonal Antibody; PCNA Mouse Monoclonal Antibody; anti-PCNA antibody

• Clonality: Monoclonal
• Clone Number: 12D10
• Specificity: Antibody can detect endogenous PCNA protein.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:5,000; IHC: 1:200
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4) with PCNA mouse mAb (12D10) diluted at 1:5000. IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb (12D10) diluted at 1:200.)

Related Product Information for anti-PCNA antibody

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

NCBI and Uniprot Product Information

• NCBI GI #: 49456555
• NCBI GeneID: 5111
• UniProt Accession #: Q6FHF5; P12004
• Molecular Weight: 28,706 Da
• NCBI Official Full Name: PCNA, partial
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.