PARK7/DJ1 Polyclonal Antibody | anti-PARK7/DJ1 antibody

Anti-PARK7/DJ1 Antibody

Cat. Num. AAA178389

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: PARK7/DJ1; Polyclonal Antibody; Anti-PARK7/DJ1 Antibody; Protein DJ-1; CAP1; DJ 1; DJ-1; DJ1; DJ1 protein; FLJ27376; FLJ34360; FLJ92274; Oncogene DJ1; OTTHUMP00000001348; OTTHUMP00000001349; OTTHUMP00000001350; OTTHUMP00000001351; Park 7; Park7; PARK7_HUMAN; Parkinson disease (autosomal recessive early onset) 7; Parkinson disease (autosomal recessive; early onset) 7; Parkinson disease protein 7; Parkinson protein 7; Protein DJ 1; RNA binding protein regulatory subunit; RS; SP22; parkinson protein 7; anti-PARK7/DJ1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 189

• Applicable Applications for anti-PARK7/DJ1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; Immunocytochemistry Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- PARK7 Picoband antibody, MBS178389, Western blottingAll lanes: Anti PARK7 (MBS178389) at 0.5ug/mlLane 1: PANC Whole Cell Lysate at 40ugLane 2: U20S Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 20KDObserved bind size: 20KD)

Immunohistochemistry (IHC)

(Anti- PARK7 Picoband antibody, MBS178389, IHC(P)IHC(P): Mouse Pancreas Tissue)

Immunohistochemistry (IHC)

(Anti- PARK7 Picoband antibody, MBS178389, IHC(P)IHC(P): Rat Pancreas Tissue)

Immunohistochemistry (IHC)

(Anti- PARK7 Picoband antibody, MBS178389, IHC(P)IHC(P): Human Pancreatic Cancer Tissue)

Immunocytochemistry (ICC)

(Anti- PARK7 Picoband antibody, MBS178389,ICCICC: A549 Cell )

Related Product Information for anti-PARK7/DJ1 antibody

Description: Rabbit IgG polyclonal antibody for Protein DJ-1(PARK7) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Background: Parkinson disease (autosomal recessive, early onset) 7, also known as DJ1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. PARK7 is mapped to chromosome 1p36. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. This gene may also function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that PARK7 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease.

References

1. Bonifati, V., Rizzu, P., van Baren, M. J., Schaap, O., Breedveld, G. J., Krieger, E., Dekker, M. C. J., Squitieri, F., Ibanez, P., Joosse, M., van Dongen, J. W., Vanacore, N., van Swieten, J. C., Brice, A., Meco, G., van Duijn, C. M., Oostra, B. A., Heutink, P. Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism. Science 299: 256-259, 2003. 2. Ottolini, D., Cali, T., Negro, A., Brini, M. The Parkinson disease-related protein DJ-1 counteracts mitochondrial impairment induced by the tumour suppressor protein p53 by enhancing endoplasmic reticulum-mitochondria tethering. Hum. Molec. Genet. 22: 2152-2168, 2013. 3. Xu, J., Zhong, N., Wang, H., Elias, J. E., Kim, C. Y., Woldman, I., Pifl, C., Gygi, S. P., Geula, C., Yankner, B. A. The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum. Molec. Genet. 14: 1231-1241, 2005.

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI Accession #: NP_001116849.1
• NCBI GenBank Nucleotide #: NM_001123377.1
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein deglycase DJ-1
• NCBI Official Synonym Full Names: Parkinsonism associated deglycase
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Nuclear receptor co-regulator; Tumor suppressor; Transcription regulation; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: axon; chromatin; cytoplasm; cytosol; endoplasmic reticulum; lipid raft; mitochondrial intermembrane space; mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion; nucleus; plasma membrane; PML body

Molecular Function: androgen receptor binding; copper ion binding; cytokine binding; double-stranded DNA binding; enzyme binding; glyoxalase III activity; identical protein binding; kinase binding; mercury ion binding; mRNA binding; oxidoreductase activity, acting on peroxide as acceptor; peptidase activity; peroxiredoxin activity; protein binding; protein homodimerization activity; receptor binding; single-stranded DNA binding; superoxide dismutase copper chaperone activity; transcription coactivator activity; transcription factor binding

Biological Process: activation of protein kinase B; adult locomotory behavior; autophagy; detoxification of copper ion; detoxification of mercury ion; dopamine uptake; enzyme active site formation via L-cysteine sulfinic acid; glycolate biosynthetic process; hydrogen peroxide metabolic process; inflammatory response; lactate biosynthetic process; membrane depolarization; membrane hyperpolarization; methylglyoxal catabolic process to D-lactate; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; negative regulation of protein export from nucleus; negative regulation of protein kinase activity; negative regulation of protein sumoylation; negative regulation of protein ubiquitination; negative regulation of ubiquitin-protein ligase activity; positive regulation of interleukin-8 production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein deglycosylation; protein stabilization; proteolysis; Ras protein signal transduction; regulation of inflammatory response; regulation of mitochondrial membrane potential; regulation of neuron apoptosis; regulation of TRAIL receptor biosynthetic process; response to drug; single fertilization

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset