Sheep Rap Guanine Nucleotide Exchange Factor 1 ELISA Kit | RAPGEF1 elisa kit

Sheep Rap Guanine Nucleotide Exchange Factor 1 ELISA Kit

Cat. Num. AAA751926

• Gene Names: RAPGEF1; C3G; GRF2
• Reactivity: Sheep
• Synonyms: Rap Guanine Nucleotide Exchange Factor 1; Sheep Rap Guanine Nucleotide Exchange Factor 1 ELISA Kit; RAPGEF1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAPGEF1 elisa kit

Principle of the assay: RAPGEF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAPGEF1 antibody and an RAPGEF1-HRP conjugate. The assay sample and buffer are incubated together with RAPGEF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAPGEF1 concentration since RAPGEF1 from samples and RAPGEF1-HRP conjugate compete for the anti-RAPGEF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RAPGEF1 from the sample, fewer sites are left to bind RAPGEF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAPGEF1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38373675
• NCBI GeneID: 2889
• NCBI Accession #: NP_005303.2
• NCBI GenBank Nucleotide #: NM_005312.2
• Molecular Weight: 122,735 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 1 isoform a
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 1
• NCBI Official Symbol: RAPGEF1
• NCBI Official Synonym Symbols: C3G; GRF2
• NCBI Protein Information: rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
• UniProt Protein Name: Rap guanine nucleotide exchange factor 1
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF1
• UniProt Synonym Gene Names: GRF2
• UniProt Entry Name: RPGF1_HUMAN

NCBI Description

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

RAPGEF1: an adaptor-type Src homology (SH)2-containing molecule. A guanine nucleotide releasing protein for Ras. Expressed ubiquitously in human adult and fetal tissues. Several alternatively spliced transcript variants have been described.

Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: early endosome; cytosol; endosome

Molecular Function: protein binding; Rap guanyl-nucleotide exchange factor activity; SH3 domain binding

Biological Process: blood vessel development; cell-cell adhesion; negative regulation of protein kinase B signaling cascade; nerve growth factor receptor signaling pathway; activation of MAPKK activity; negative regulation of Ras protein signal transduction; platelet-derived growth factor receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; Rap protein signal transduction