Rabbit anti-Human PARK7 Polyclonal Antibody | anti-PARK7 antibody

PARK7 Antibody (C-term)

Cat. Num. AAA9208096

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PARK7; Polyclonal Antibody; PARK7 Antibody (C-term); Protein DJ-1; 34--; Oncogene DJ1; Parkinson disease protein 7; anti-PARK7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PARK7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 130-164 amino acids from the C-terminal region of human PARK7.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-PARK7 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC~~1:25; WB~~1:1000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. kideny section using PARK7 Antibody (C-term). MBS9208096 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysate from Hela cell line, using PARK7 Antibody (C-term). MBS9208096 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Related Product Information for anti-PARK7 antibody

Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone. May regulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells.

Product Categories/Family for anti-PARK7 antibody

Crown Antibodies; Cancer; Cell Biology; Metabolism; Signal Transduction

References

Nagakubo D.,et al.Biochem. Biophys. Res. Commun. 231:509-513(1997).
Beaudoin R.,et al.Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
Ariga H.,et al.Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI Accession #: NP_001116849.1
• NCBI GenBank Nucleotide #: NM_001123377.1
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19891
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Tumor suppressor; Nuclear receptor co-regulator; Transcription regulation; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: PML body; mitochondrion; mitochondrial matrix; axon; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; cytosol; mitochondrial respiratory chain complex I; chromatin; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; peptidase activity; mRNA binding; protein binding; enzyme binding; copper ion binding; peroxiredoxin activity; androgen receptor binding; cytokine binding; double-stranded DNA binding; glyoxalase III activity; kinase binding; superoxide dismutase copper chaperone activity; single-stranded DNA binding; receptor binding

Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; methylglyoxal catabolic process to D-lactate; positive regulation of protein kinase B signaling cascade; membrane depolarization; detoxification of mercury ion; regulation of inflammatory response; Ras protein signal transduction; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; autophagy; lactate biosynthetic process; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; negative regulation of apoptosis

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset