Mouse PARK7 / DJ-1 Monoclonal Antibody | anti-PARK7 / DJ-1 antibody

Anti-PARK7 / DJ-1 Antibody (clone 4H4) IHC-plus

Cat. Num. AAA249405

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Mouse, Bovine, Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: PARK7 / DJ-1; Monoclonal Antibody; Anti-PARK7 / DJ-1 Antibody (clone 4H4) IHC-plus; Mouse Monoclonal [clone 4H4] (IgG1; k) to Human PARK7 / DJ-1; Human PARK7 / DJ-1; DJ1; Parkinson disease protein 7; Park-7; Oncogene DJ1; Parkinson protein 7; Protein DJ-1; anti-PARK7 / DJ-1 antibody

• Host: Mouse
• Reactivity: Mouse, Bovine, Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4H4
• Specificity: Clone 4H4 recognizes DJ-1 specifically both in western blots and in immunocytochemical experiments. On blots, clone 4H4 reveals a prominent ~21 kDa band, and on cells in tissue culture the antibody stains mainly in cytoplasm. Clone 4H4 has been tested to react with DJ-1 from human, cow, mouse.
• Purity/Purification: Affinity purified
• Form/Format: PBS, 10 mM sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-PARK7 / DJ-1 antibody: Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: ICC (1:5000); IF (1:5000); IHC-P (10 ug/ml); WB (1:5000)
• Immunogen: PARK7 / DJ-1 antibody was raised against mCA-4H4 was generated against full length recombinant human DJ-1 expressed in and purified from E Coli.
• Immunogen Description: MCA-4H4 was generated against full length recombinant human DJ-1 expressed in and purified from E Coli.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: 4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry - Paraffin (IHC)

(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunocytochemistry (ICC)

(HeLa cells stained with PARK7 / DJ-1 antibody (green), and chicken antibody to vimentin (red) and DNA (blue). PARK7 / DJ-1 antibody antibody reveals strong cytoplasmic staining for DJ-1.)

Western Blot (WB)

(Western blot of whole HeLa cell lysates probed with PARK7 / DJ-1 antibody antibody to DJ-1. Note that the strong single band running at about 21kDa corresponds to DJ-1.)

Related Product Information for anti-PARK7 / DJ-1 antibody

PARK7 Antibody, DJ-1 Antibody, DJ1 Antibody, Parkinson disease protein 7 Antibody, Park-7 Antibody, Oncogene DJ1 Antibody, Parkinson protein 7 Antibody, Protein DJ-1 Antibody Description: Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 31543380
• NCBI GeneID: 11315
• NCBI Accession #: NP_009193.2
• NCBI GenBank Nucleotide #: NM_007262.4
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: Parkinsonism associated deglycase
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Nuclear receptor co-regulator; Tumor suppressor; Transcription regulation; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: axon; chromatin; cytoplasm; cytosol; endoplasmic reticulum; lipid raft; mitochondrial intermembrane space; mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion; nucleus; plasma membrane; PML body

Molecular Function: androgen receptor binding; copper ion binding; cytokine binding; double-stranded DNA binding; enzyme binding; glyoxalase III activity; identical protein binding; kinase binding; mercury ion binding; mRNA binding; oxidoreductase activity, acting on peroxide as acceptor; peptidase activity; peroxiredoxin activity; protein binding; protein homodimerization activity; receptor binding; single-stranded DNA binding; superoxide dismutase copper chaperone activity; transcription coactivator activity; transcription factor binding

Biological Process: activation of protein kinase B; adult locomotory behavior; autophagy; detoxification of copper ion; detoxification of mercury ion; dopamine uptake; enzyme active site formation via L-cysteine sulfinic acid; glycolate biosynthetic process; hydrogen peroxide metabolic process; inflammatory response; lactate biosynthetic process; membrane depolarization; membrane hyperpolarization; methylglyoxal catabolic process to D-lactate; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; negative regulation of protein export from nucleus; negative regulation of protein kinase activity; negative regulation of protein sumoylation; negative regulation of protein ubiquitination; negative regulation of ubiquitin-protein ligase activity; positive regulation of interleukin-8 production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein deglycosylation; protein stabilization; proteolysis; Ras protein signal transduction; regulation of inflammatory response; regulation of mitochondrial membrane potential; regulation of neuron apoptosis; regulation of TRAIL receptor biosynthetic process; response to drug; single fertilization

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset