Rabbit DJ-1 Polyclonal Antibody | anti-DJ-1 antibody

DJ-1 Antibody

Cat. Num. AAA9407576

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: DJ-1; Polyclonal Antibody; DJ-1 Antibody; ONCOGENE DJ1; PARK7 AMYOTROPHIC LATERAL SCLEROSIS-PARKINSONISM/DEMENTIA COMPLEX 2; PARK7 GENE; anti-DJ-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total DJ-1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-DJ-1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from human DJ-1.
• Target Name: DJ-1
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using DJ-1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using DJ-1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HuvEc cells, using DJ-1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using DJ-1 antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-DJ-1 antibody

Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone. May regulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells.Nobutaka Hattori, Hum. Mol. Genet., Jan 2005; 14: 71 - 84.Raymond H. Kim, PNAS, Apr 2005; 102: 5215 - 5220.Rina Bandopadhyay, Brain, Feb 2004; 127: 420 - 430.Nan Zhong, J. Biol. Chem., Jul 2006; 281: 20940 - 20948.

Product Categories/Family for anti-DJ-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI Accession #: NP_001116849.1
• NCBI GenBank Nucleotide #: NM_001123377.1
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• Protein Family: DJ-1 protein
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: EC 3.4.-.-; Tumor suppressor; Nuclear receptor co-regulator; Transcription regulation

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: PML body; mitochondrion; axon; mitochondrial matrix; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; mitochondrial respiratory chain complex I; cytosol; chromatin; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; mRNA binding; peptidase activity; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; kinase binding; superoxide dismutase copper chaperone activity; single-stranded DNA binding; receptor binding

Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; methylglyoxal catabolic process to D-lactate; membrane depolarization; positive regulation of protein kinase B signaling cascade; detoxification of mercury ion; Ras protein signal transduction; regulation of inflammatory response; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; autophagy; lactate biosynthetic process; negative regulation of protein kinase activity; positive regulation of transcription factor activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset