Rabbit anti-Human PAK6 Polyclonal Antibody | anti-PAK6 antibody

PAK6, ID (PAK6, PAK5, Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6)

Cat. Num. AAA6005391

• Gene Names: PAK6; PAK5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PAK6; Polyclonal Antibody; ID (PAK6; PAK5; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6); Anti -PAK6; anti-PAK6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PAK6 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Immunofluorescence: 1:10-50
• Immunogen: PAK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-305 amino acids from the Central region of human PAK6.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).)

Immunohistochemistry (IHC)

(PAK6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PAK6 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PAK6 antibody

This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-PAK6 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 23272546
• NCBI GeneID: 56924
• UniProt Accession #: Q9NQU5; A8K2G2
• Molecular Weight: 74,869 Da
• NCBI Official Full Name: PAK6 protein
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 6
• NCBI Official Symbol: PAK6
• NCBI Official Synonym Symbols: PAK5
• NCBI Protein Information: serine/threonine-protein kinase PAK 6; p21(CDKN1A)-activated kinase 6
• UniProt Protein Name: Serine/threonine-protein kinase PAK 6
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK6
• UniProt Synonym Gene Names: PAK5; PAK-6
• UniProt Entry Name: PAK6_HUMAN

NCBI Description

This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

PAK6: a protein kinase of the STE20 family. Interacts with androgen receptor (AR), and cotranslocates into the nucleus with AR in response to androgen. Highly expressed in testis and prostate tissues.

Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: regulation of transcription, DNA-dependent; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; protein amino acid phosphorylation