Rat Apoptosis Signal Regulating Kinase 1 (ASK1) ELISA Kit | ASK1 elisa kit

Rat Apoptosis Signal Regulating Kinase 1 (ASK1) ELISA Kit

Cat. Num. AAA260107

• Gene Names: MAP3K5; ASK1; MEKK5; MAPKKK5
• Reactivity: Rat
• Synonyms: Apoptosis Signal Regulating Kinase 1 (ASK1); Rat Apoptosis Signal Regulating Kinase 1 (ASK1) ELISA Kit; ASK1 elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1374

• Samples: Rat serum, plasma or cell culture supernatant and organizations
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASK1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat ASK1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 66391167
• NCBI GeneID: 4217
• Molecular Weight: 154,537 Da
• NCBI Official Full Name: apoptosis signal-regulating kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Symbol: MAP3K5
• NCBI Official Synonym Symbols: ASK1; MEKK5; MAPKKK5
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 5; ASK-1; MEKK 5; MEK kinase 5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 5
• Protein Family: DASH complex
• UniProt Gene Name: MAP3K5
• UniProt Synonym Gene Names: ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5
• UniProt Entry Name: M3K5_HUMAN

NCBI Description

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Uniprot Description

ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: cytosol

Molecular Function: protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; magnesium ion binding; caspase activator activity; protein kinase binding; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: caspase activation; positive regulation of JNK activity; viral reproduction; activation of MAPKK activity; positive regulation of apoptosis; MAPKKK cascade; positive regulation of caspase activity; positive regulation of JNK cascade; protein amino acid phosphorylation; activation of JNK activity; innate immune response; JNK cascade; induction of apoptosis by oxidative stress