Western Blot (WB)
(PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).)
Rabbit anti-Human PAK6 Polyclonal Antibody | anti-PAK6 antibody
PAK6, ID (PAK6, PAK5, Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6) (APC)
Gene Names
PAK6; PAK5; PAK6
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PAK6; Polyclonal Antibody; ID (PAK6; PAK5; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6) (APC); p21-activated kinase 6; anti-PAK6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PAK6 antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PAK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-305 amino acids from the Central region of human PAK6.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).)
Western Blot (WB)
(PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).)
Immunohistochemistry (IHC)
(PAK6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PAK6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PAK6 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PAK6 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-PAK6 antibody
This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Product Categories/Family for anti-PAK6 antibody
References
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Lee, E.J., et al. Oncol. Res. 18(9):401-408(2010). Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009). Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein kinase PAK 6 [Homo sapiens]
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 6
NCBI Official Symbol
PAK6
NCBI Official Synonym Symbols
PAK5; PAK6
UniProt Protein Name
Serine/threonine-protein kinase PAK 6
Protein Family
UniProt Gene Name
PAK6
UniProt Synonym Gene Names
PAK5; PAK-6
UniProt Entry Name
PAK6_HUMAN
Uniprot Description
PAK6: a protein kinase of the STE20 family. Interacts with androgen receptor (AR), and cotranslocates into the nucleus with AR in response to androgen. Highly expressed in testis and prostate tissues.
Protein type: Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; EC 2.7.11.1; STE group; STE20 family; PAKB subfamily
Chromosomal Location of Human Ortholog: 15q14
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: regulation of transcription, DNA-dependent; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; protein amino acid phosphorylation
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Product Notes
The PAK6 pak6 (Catalog #AAA6330583) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAK6, ID (PAK6, PAK5, Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAK6 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PAK6 pak6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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