Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase (Phospho-Ser411) antibody)
Rabbit p70S6 Kinase Polyclonal Antibody
p70S6 Kinase antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
p70S6 Kinase antibody was purified by immunoaffinity chromatography.
Synonyms
p70S6 Kinase; Polyclonal Antibody; p70S6 Kinase antibody; Polyclonal p70S6 Kinase; Anti-p70S6 Kinase; pS6 Kinase-70; p70S6K; Phospho Ser411; pS6 Kinase 70; anti-p70S6 Kinase antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
p70S6 Kinase antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-p70S6 Kinase antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Biological Significance
p70S6 kinase is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. As the name suggests, its target substrate is the S6 ribosomal protein. Phosphorylation of S6 induces protein synthesis at the ribosome.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
p70S6 Kinase antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase (Phospho-Ser411) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase (Phospho-Ser411) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser411) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser411) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser411) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser411) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-p70S6 Kinase antibody
Rabbit polyclonal p70S6 Kinase antibody
Product Categories/Family for anti-p70S6 Kinase antibody
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Product Notes
The p70S6 Kinase (Catalog #AAA837318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p70S6 Kinase can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the p70S6 Kinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p70S6 Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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