Western Blot (WB)
(Western blot analysis of p70 S6 kinase alpha phosphorylation in untreated (A, D), insulin treated (B, E) and insulin treated and lambda protein phosphatase treated (C, F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase alpha (A-6) (A, B, C) and p70 S6 kinase alpha (C-18) (D, E, F).)
Mouse p70S6 Kinase Monoclonal Antibody | anti-RPS6KB2 antibody
p70 S6 Kinase (Phospho-Ser 411) Antibody
IHC: 1:50-1:500
IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
Western Blot (WB)
(Western blot analysis of p70 S6 kinase alpha phosphorylation in untreated (A, D), insulin treated (B, E) and insulin treated and lambda protein phosphatase treated (C, F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase alpha (A-6) (A, B, C) and p70 S6 kinase alpha (C-18) (D, E, F).)
Western Blot (WB)
(Western blot analysis of p70 S6 kinase alpha phosphorylation in untreated (A, D), insulin treated (B, E) and insulin treated and lambda protein phosphatase treated (C, F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase alpha (A-6) (A, B, C) and p70 S6 kinase alpha (C-18) (D, E, F).)
Immunofluorescence (IF)
(Immunofluorescence staining of anisomycin-treated, methanol-fixed NIH/3T3 cells, showing cytoplasmic and nuclear localization of activated p70 S6 kinase alpha (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells (B).)
Immunofluorescence (IF)
(Immunofluorescence staining of anisomycin-treated, methanol-fixed NIH/3T3 cells, showing cytoplasmic and nuclear localization of activated p70 S6 kinase alpha (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells (B).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jul 2008]
Uniprot Description
P70S6KB: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.
Protein type: Protein kinase, AGC; EC 2.7.11.1; Translation; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; RSK family; p70 subfamily
Chromosomal Location of Human Ortholog: 11q13.2
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein serine/threonine kinase activity; ribosomal protein S6 kinase activity; peptide binding; ATP binding; protein kinase activity
Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of translational initiation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; translation; nerve growth factor receptor signaling pathway; protein kinase B signaling cascade; innate immune response; signal transduction; protein amino acid phosphorylation
Research Articles on RPS6KB2
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Product Notes
The RPS6KB2 rps6kb2 (Catalog #AAA9430990) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The p70 S6 Kinase (Phospho-Ser 411) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p70S6 Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. WB: 1:100-1:1000 IHC: 1:50-1:500 IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate). Researchers should empirically determine the suitability of the RPS6KB2 rps6kb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p70S6 Kinase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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