Rabbit NFKB p105 Polyclonal Antibody | anti-NFKB p105 antibody

NFKB p105 antibody

Cat. Num. AAA835669

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: NFKB p105 antibody was purified by immunoaffinity chromatography.
• Synonyms: NFKB p105; Polyclonal Antibody; NFKB p105 antibody; Polyclonal NFKB p105; Anti-NFKB p105; Phospho Ser932; DNA binding factor KBF1; NFKB p-105; DNA-binding factor KBF1; p50; EBP-1; MGC54151; NF-kappaB; NF-kappa-B; DKFZp686C01211; NFKB p 105; Phospho Ser927; KBF1; anti-NFKB p105 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: NFKB p105 antibody was purified by immunoaffinity chromatography.
• Form/Format: Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 969

• Applicable Applications for anti-NFKB p105 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100-1:200; IHC: 1:50-1:100; WB: 1:500-1:1000
• Biological Significance: NFkB-p105 a transcription factor of the nuclear factor-kappaB (NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.
• Cross-Reactivity: Human, Mouse, Rat
• Immunogen: NFKB p105 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 927 (C-D-S(p)-G-V) derived from Human NFKB-p105 as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using NFkappaB-p105/p50(Phospho-Ser927) antibody)

Western Blot (WB)

(Western Blot analysis of extracts from HL60 cells untreated or treated with TNF-alpha using NFkappaB-p105/p50 (Phospho-Ser927) antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkappaB-p105/p50 (Phospho-Ser927) antibody (left) or the same antibody preincubated with with blocking peptide (right))

Related Product Information for anti-NFKB p105 antibody

Rabbit polyclonal NFKB p105 antibody

Product Categories/Family for anti-NFKB p105 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 34577122
• NCBI GeneID: 4790
• NCBI Accession #: NP_003989.2
• NCBI GenBank Nucleotide #: NM_003998.3
• UniProt Accession #: P19838; Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
• Molecular Weight: 85,520 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 1
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN

NCBI Description

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of cholesterol transport; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; negative regulation of interleukin-12 biosynthetic process; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to copper ion; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; toll-like receptor 9 signaling pathway; negative regulation of apoptosis