Rabbit p70 S6 Kinase Polyclonal Antibody | anti-RPS6KB1 antibody

p70 S6 Kinase (Phospho-Ser411) Antibody

Cat. Num. AAA9409779

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: p70 S6 Kinase; Polyclonal Antibody; p70 S6 Kinase (Phospho-Ser411) Antibody; KS6B1; P70-S6K; RPS6KB1; S6K; anti-RPS6KB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 411.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 502

• Applicable Applications for anti-RPS6KB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase.
• Modification: Phospho-Ser411
• Target Name: p70 S6 Kinase
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser411) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser411) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Phospho-Ser411) Antibody.)

Related Product Information for anti-RPS6KB1 antibody

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Product Categories/Family for anti-RPS6KB1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 440546395
• NCBI GeneID: 6198
• NCBI Accession #: NP_001258971.1
• NCBI GenBank Nucleotide #: NM_001272042.1
• UniProt Accession #: P23443; Q7Z721; B2R779; B4DLT4; B4DTG1; E7ESB8; F6UYM1
• Molecular Weight: 51,016 Da
• NCBI Official Full Name: ribosomal protein S6 kinase beta-1 isoform b
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KB1
• UniProt Synonym Gene Names: STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
• UniProt Entry Name: KS6B1_HUMAN

Uniprot Description

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: Translation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 17q23.1