<
Western Blot (WB)
Rabbit p65 Polyclonal Antibody | anti-p65 antibody
Anti-p65 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
p65; Polyclonal Antibody; Anti-p65 Antibody; anti-p65 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Western blot analysis of extracts of Jurkat cell line, using p65 antibody. Immunohistochemistry of paraffin-embedded rat kidney using RELA antibody at dilution of 1:200 (400x lens). Immunohistochemistry of paraffin-embedded rat lung using RELA antibody at dilution of 1:200 (400x lens). Immunofluorescence analysis of U2OS cell using P65 antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
133
Applicable Applications for anti-p65 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50- 1:200
IF: 1:50- 1:200
IHC: 1:50- 1:200
IF: 1:50- 1:200
Immunogen
Recombinant protein of human p65
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
Western Blot (WB)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunofluorescence (IF)
Related Product Information for anti-p65 antibody
Transcription factors of the nuclear factor B (NF-B)/Rel family play a pivotal role in inflammatory and immune responses (1, 2). There are five family members in mammals: RelA, c-Rel, RelB, NF-B1 (p105/p50), and NF-B2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-B is sequestered in the cytoplasm by IB inhibitory proteins (3-5). NF-B-activating agents can induce the phosphorylation of IB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-B to enter the nucleus where it regulates gene expression (6-8). NIK and IKK+/- (IKK1) regulate the phosphorylation and processing of NF-B2 (p100) to produce p52, which is then translocated to the nucleus (9-11). NF-B assembly with IB, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-B (12-14).
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
15,100 Da
NCBI Official Full Name
p65, partial
UniProt Protein Name
p65
Protein Family
Similar Products
Product Notes
The p65 (Catalog #AAA4150101) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p65 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50- 1:200 IF: 1:50- 1:200. Researchers should empirically determine the suitability of the p65 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
