Rabbit p65 Polyclonal Antibody | anti-p65 antibody

p65 Polyclonal Antibody

Cat. Num. AAA8527331

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: p65; Polyclonal Antibody; p65 Polyclonal Antibody; RELA; MGC131774; NFKB3; anti-p65 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 548

• Applicable Applications for anti-p65 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:20-1:50; IP 1:20-1:50
• Immunogen: Fusion protein of human p65
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of HeLa celland A431 cell line, using p65 antibody.)

Related Product Information for anti-p65 antibody

Transcription factors of the nuclear factor kappa B (NF-kappaB)/Rel family play a pivotal role in inflammatory and immune responses (1, 2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kappaB1 (p105/p50), and NF-kappaB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kappaB is sequestered in the cytoplasm by IkappaB inhibitory proteins (3-5). NF-kappaB-activating agents can induce the phosphorylation of IkappaB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kappaB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kappaB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). NF-kappaB assembly with IkappaB, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-kappaB (12-14).

References

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3. Haskill, S. et al. (1991) Cell 65, 1281-9. 4. Thompson, J.E. et al. (1995) Cell 80, 573-82. 5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8. Chen, Z.J. et al. (1996) Cell 84, 853-62. 9. Senftleben, U. et al. (2001) Science 293, 1495-9. 10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11. Xiao, G. et al. (2001) Mol Cell 7, 401-9. 12. Ashburner, B.P. et al. (2001) Mol. Cell. Biol. 21, 7065-7077. 13. Mayo, M.W. et al. (2003) J. Biol. Chem. 278, 18980-1899. 14. Chen, L.F. et al. (2002) EMBO J. 21, 6539-6548.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 65kDa
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: RELA proto-oncogene, NF-kB subunit
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• Protein Family: Proline-rich P65 protein
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).