Western Blot (WB)
(Sample Data: Western blot analysis using Anti-Cyclin D1 (1:500) to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1.)
anti-Human Cyclin D1 Polyclonal Antibody
Anti-Cyclin D1
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Delipidation and defibrination
Synonyms
Cyclin D1; Polyclonal Antibody; Anti-Cyclin D1; anti-Cyclin D1 antibody
Host
Rabbit, Antiserum
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes the 40-45kDa Cyclin D1 protein
Purity/Purification
Delipidation and defibrination
Form/Format
0.01% (w/v) Sodium Azide
Applicable Applications for anti-Cyclin D1 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
The antibody was produced against full length fusion protein corresponding to the human Cyclin D1 gene sequence.
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis using Anti-Cyclin D1 (1:500) to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1.)
Western Blot (WB)
(Sample Data: Western blot analysis using Anti-Cyclin D1 (1:500) to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1.)
Related Product Information for anti-Cyclin D1 antibody
Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Product Categories/Family for anti-Cyclin D1 antibody
References
1. Xiong, Y., et al.: Human D-type cyclin. Cell. 1991: 65; 691-699.
2. Motokura, T., et al.: A novel cyclin encoded by a bcl1-linked candidate oncogene. Nature. 1991: 350; 512-515.
3. Lutzen,A., et al.: Cyclin D1 expression and cell cycle response in DNA mismatch repair-deficient cells upon methylation and UV-C damage. Exp. Cell Res. 2004: 292 (1); 123-134.
4. Pines J.: Cyclins and cyclin-dependent kinases: take your partners. Trends Biochem Sci. 1993: 18(6);195-7.
5. Xiong, Y., et al.: Human D-type cyclin. Cell. 1991: 65; 691-699.
2. Motokura, T., et al.: A novel cyclin encoded by a bcl1-linked candidate oncogene. Nature. 1991: 350; 512-515.
3. Lutzen,A., et al.: Cyclin D1 expression and cell cycle response in DNA mismatch repair-deficient cells upon methylation and UV-C damage. Exp. Cell Res. 2004: 292 (1); 123-134.
4. Pines J.: Cyclins and cyclin-dependent kinases: take your partners. Trends Biochem Sci. 1993: 18(6);195-7.
5. Xiong, Y., et al.: Human D-type cyclin. Cell. 1991: 65; 691-699.
Similar Products
Product Notes
The Cyclin D1 (Catalog #AAA515923) is an Antibody produced from Rabbit, Antiserum and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin D1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin D1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Cyclin D1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
