Western Blot (WB)
(Sample Data: Western blot analysis is shown using Anti-NEK2 (1:500 dilution) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead).)
Rabbit anti-Human NEK2 Polyclonal Antibody | anti-NEK2 antibody
Anti-NEK2
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Affinity chromatography
Synonyms
NEK2; Polyclonal Antibody; Anti-NEK2; anti-NEK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes human and chimpanzee NEK2 homologues
Purity/Purification
Affinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-NEK2 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 287-299 of human NEK2 protein
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis is shown using Anti-NEK2 (1:500 dilution) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead).)
Western Blot (WB)
(Sample Data: Western blot analysis is shown using Anti-NEK2 (1:500 dilution) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead).)
Related Product Information for anti-NEK2 antibody
NEK 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2 (1). NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus (2).
Product Categories/Family for anti-NEK2 antibody
References
1. Schultz, SJ. et al: A. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. Cell Growth Differ. 1994 Jun;5(6):625-35.
2. Fry, AM. et al: Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans. J Biol Chem. 1995 May 26; 270(21):12899-905.
2. Fry, AM. et al: Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans. J Biol Chem. 1995 May 26; 270(21):12899-905.
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Product Notes
The NEK2 (Catalog #AAA516109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NEK2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the NEK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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