Western Blot (WB)
(Western blot analysis of p27 Kip1 (pT198) expression in HEK293T EGF-treated (A); MCF7 (B); NIH3T3 (C); Raw264.7 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.)
Rabbit p27 Kip1 Polyclonal Antibody | anti-p27Kip1 antibody
p27 Kip1 Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Applications
Western Blot
Purity
Immunogen Affinity Chromatography
Synonyms
p27 Kip1; Polyclonal Antibody; p27 Kip1 Antibody; Purified Polyclonal p27 Kip1 Antibody; Polyclonal p27 Kip1 antibody; Anti-p27 Kip1 antibody; KIP1 antibody; Cyclin-dependent kinase inhibitor 1B antibody; Cyclin-dependent kinase inhibitor p27 antibody; p27Kip1 antibody; anti-p27Kip1 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of p27 Kip1 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
158
Applicable Applications for anti-p27Kip1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
p27 Kip1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p27 Kip1 as the immunogen
Cross-Reactivity
Mouse, Rat, Bovine, Dog, Pig, Rabbit
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of p27 Kip1 (pT198) expression in HEK293T EGF-treated (A); MCF7 (B); NIH3T3 (C); Raw264.7 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p27 Kip1 (pT198) expression in HEK293T EGF-treated (A); MCF7 (B); NIH3T3 (C); Raw264.7 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.)
Related Product Information for anti-p27Kip1 antibody
p27 Kip1 is a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1.
Product Categories/Family for anti-p27Kip1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
p27 kip1, partial
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 1B
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B
NCBI Description
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
Research Articles on p27Kip1
Similar Products
Product Notes
The p27Kip1 (Catalog #AAA5314147) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p27 Kip1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the p27Kip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p27 Kip1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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