Western Blot (WB)
(Western blot detection of p21 in Raw264.7 cell lysates using p21 Rabbit pAb(1:1000 diluted). Predicted band size:18KDa. Observed band size:18KDa.)
Rabbit anti-Mouse p21 Polyclonal Antibody | anti-Cdkn1a antibody
p21 Rabbit pAb
Gene Names
Cdkn1a; P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP20; Cdkn1; p21WAF; p21Cip1
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
p21; Polyclonal Antibody; p21 Rabbit pAb; anti-Cdkn1a antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-Cdkn1a antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:200
ICC/IF: 1:100
FC/FACS: 1:20
IP: 1:20
IHC: 1:200
ICC/IF: 1:100
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of mouse p21
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of p21 in Raw264.7 cell lysates using p21 Rabbit pAb(1:1000 diluted). Predicted band size:18KDa. Observed band size:18KDa.)
Western Blot (WB)
(Western blot detection of p21 in Raw264.7 cell lysates using p21 Rabbit pAb(1:1000 diluted). Predicted band size:18KDa. Observed band size:18KDa.)
Related Product Information for anti-Cdkn1a antibody
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed:25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
17,785 Da
NCBI Official Full Name
p21
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1A (P21)
NCBI Official Symbol
Cdkn1a
NCBI Official Synonym Symbols
P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP20; Cdkn1; p21WAF; p21Cip1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1; p21<WAF1/CIP1>; CDK-interacting protein 1; melanoma differentiation-associated protein
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1
Protein Family
UniProt Gene Name
Cdkn1a
UniProt Synonym Gene Names
Cip1; Waf1
UniProt Entry Name
CDN1A_MOUSE
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Product Notes
The Cdkn1a cdkn1a (Catalog #AAA4751756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p21 Rabbit pAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:200 ICC/IF: 1:100 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the Cdkn1a cdkn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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