Rabbit GCLC Polyclonal Antibody | anti-GCLC antibody

GCLC Rabbit Polyclonal

Cat. Num. AAA769618

• Gene Names: GCLC; GCL; GCS; GLCL; GLCLC
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: GCLC; Polyclonal Antibody; GCLC Rabbit Polyclonal; anti-GCLC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 599

• Applicable Applications for anti-GCLC antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Glutamate-cysteine ligase, catalytic subunit
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS769618 (GCLC antibody) at dilution of 1:50)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS769618 (GCLC antibody) at dilution of 1:2000)

NCBI and Uniprot Product Information

• NCBI GI #: 308199423
• NCBI GeneID: 2729
• NCBI Accession #: NP_001184044.1
• NCBI GenBank Nucleotide #: NM_001197115.1
• Molecular Weight: 72,766 Da
• NCBI Official Full Name: glutamate--cysteine ligase catalytic subunit isoform b
• NCBI Official Synonym Full Names: glutamate-cysteine ligase catalytic subunit
• NCBI Official Symbol: GCLC
• NCBI Official Synonym Symbols: GCL; GCS; GLCL; GLCLC
• NCBI Protein Information: glutamate--cysteine ligase catalytic subunit
• UniProt Protein Name: Glutamate--cysteine ligase catalytic subunit
• Protein Family: Glutamate--cysteine ligase
• UniProt Gene Name: GCLC
• UniProt Synonym Gene Names: GLCL; GLCLC
• UniProt Entry Name: GSH1_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

Uniprot Description

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.

Protein type: Other Amino Acids Metabolism - glutathione; Ligase; EC 6.3.2.2

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: cytosol; glutamate-cysteine ligase complex

Molecular Function: ADP binding; ATP binding; coenzyme binding; glutamate binding; glutamate-cysteine ligase activity; magnesium ion binding; protein heterodimerization activity

Biological Process: apoptotic mitochondrial changes; cell redox homeostasis; cysteine metabolic process; glutamate metabolic process; glutathione biosynthetic process; L-ascorbic acid metabolic process; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of protein ubiquitination; negative regulation of transcription, DNA-dependent; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of blood vessel size; regulation of mitochondrial depolarization; response to arsenic; response to heat; response to hormone stimulus; response to nitrosative stress; response to oxidative stress; response to xenobiotic stimulus; sulfur amino acid metabolic process

Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To