Testing Data
(Human FFPE collon carcinoma stained with P1000-54 and AEC chromagen)
Mouse anti-Human p21, WAF1 Monoclonal Antibody | anti-Cdkn1a antibody
p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein) (BSA & Azide Free)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunoprecipitation (native and denatured) : 2ug/ml protein lysate. Co-precipitates p21 proteins.
Immunohistochemistry (formalin and paraffin): 1:25-1:50 for 60 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Testing Data
(Human FFPE collon carcinoma stained with P1000-54 and AEC chromagen)
Testing Data
(Human FFPE collon carcinoma stained with P1000-54 and AEC chromagen)
NCBI and Uniprot Product Information
Uniprot Description
p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.
Protein type: Inhibitor; Cell cycle regulation
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus
Molecular Function: cyclin-dependent protein kinase inhibitor activity; cyclin binding; protein binding; cyclin-dependent protein kinase activating kinase activity; protein kinase inhibitor activity; metal ion binding; ubiquitin protein ligase binding; protein complex binding
Biological Process: regulation of cell cycle; regulation of mitotic cell cycle; negative regulation of cyclin-dependent protein kinase activity; cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of programmed cell death; positive regulation of fibroblast proliferation; positive regulation of protein kinase activity; positive regulation of B cell proliferation; cellular response to extracellular stimulus; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; cell cycle arrest; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to UV; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Research Articles on Cdkn1a
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Product Notes
The Cdkn1a cdkn1a (Catalog #AAA602466) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein) (BSA & Azide Free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p21, WAF1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Immunoprecipitation and Immunohistology. Not suitable for Flow Cytometry or Western Blot. Dilution: Immunoprecipitation (native and denatured) : 2ug/ml protein lysate. Co-precipitates p21 proteins. Immunohistochemistry (formalin and paraffin): 1:25-1:50 for 60 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Researchers should empirically determine the suitability of the Cdkn1a cdkn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21, WAF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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