anti-Human ALK3 / BMPR1A Monoclonal Antibody | anti-ALK3 antibody

Mouse Monoclonal [clone 4B7B2] (IgG1) to Human ALK3 / BMPR1A

Cat. Num. AAA249195

• Gene Names: BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, ELISA
• Purity: Ascites
• Synonyms: ALK3 / BMPR1A; Monoclonal Antibody; Mouse Monoclonal [clone 4B7B2] (IgG1) to Human ALK3 / BMPR1A; Anti-ALK3 / BMPR1A Antibody (aa179-378; clone 4B7B2) IHC-plus; BMPR1A; 10q23del; ALK3; ALK-3; BMP type-1A receptor; BMPR-1A; CD292; CD292 antigen; Activin receptor-like kinase 3; ACVRLK3; SKR5; Human ALK3; anti-ALK3 antibody

• Host: ALK3/BMPR1A antibody was produced in Mouse.
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4B7B2
• Specificity: Human, Mouse
• Purity/Purification: Ascites
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 532

• Applicable Applications for anti-ALK3 antibody: IHC, IHC-Paraffin, Immunofluorescence, Western Blot, Flow Cytometry, ELISA.
• Application Notes: IHC-Paraffin (1:50); Immunofluorescence (1:200-1:1000); Western Blot (1:100-1:500); Flow Cytometry (1:200-1:400); ELISA (1:1000); ; Optimal dilution to be determined by the researcher.
• Target: ALK3/ BMPR1A
• Family/Subfamily: Protein Kinase/ Activin/TGFb
• Immunogen Species: ALK3/BMPR1A antibody was raised against Human
• Immunogen Description: ALK3/BMPR1A antibody was raised against purified recombinant fragment of human BMPR1A (AA:179-378)expressed in E.Coli).
• Epitope: aa179-378
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Short term: 4°C ; Long term: -20°C.

Immunohistochemistry (IHC)

(Anti-ALK3 / BMPR1A antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells using BMPR1A mouse mAb (green) and negative control (purple).)

Western Blot (WB)

(Western blot of BMPR1A mouse mAb against PC-3 (1), K562 (2) cell lysate, and Mouse liver (3) tissue lysate.)

Western Blot (WB)

(Western blot of BMPR1A mAb against HEK293 (1) and BMPR1A (AA: 179-378)-hIgGFc transfected HEK293 (2) cell lysate.)

ELISA (EIA)

(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

Related Product Information for anti-ALK3 antibody

On ligand binding of BMP-2 and BMP-4, BMPR1A (a type I receptor) forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Targeted disruption of the BMPR1A receptor protein in the mesenchymal cells of the Mullerian ducts of mice leads to retention of oviducts and uteri in males.

Product Categories/Family for anti-ALK3 antibody

Family: Protein Kinase; Subfamily: Activin/TGFb

NCBI and Uniprot Product Information

• NCBI GI #: 41349437
• NCBI GeneID: 657
• NCBI Accession #: NP_004320.2
• NCBI GenBank Nucleotide #: NM_004329.2
• UniProt Accession #: P36894; Q8NEN8; A8K6U9
• Molecular Weight: 60,198 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1A
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type IA
• NCBI Official Symbol: BMPR1A
• NCBI Official Synonym Symbols: ALK3; SKR5; CD292; ACVRLK3; 10q23del
• NCBI Protein Information: bone morphogenetic protein receptor type-1A; ALK-3; BMPR-1A; BMP type-1A receptor; activin receptor-like kinase 3; activin A receptor, type II-like kinase 3; serine/threonine-protein kinase receptor R5
• UniProt Protein Name: Bone morphogenetic protein receptor type-1A
• UniProt Gene Name: BMPR1A
• UniProt Synonym Gene Names: ACVRLK3; ALK3; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5
• UniProt Entry Name: BMR1A_HUMAN

NCBI Description

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq, Jul 2008]

Uniprot Description

BMPR1A: a serine/threonine-protein kinase receptor for Bone morphogenetic protein-2 and -4 (BMP-2 and BMP-4). Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) and Cowden disease (CD), a cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometriel cancers.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; EC 2.7.11.30; Kinase, protein; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: cell soma; dendrite; integral to membrane; plasma membrane; caveola

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; metal ion binding; SMAD binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: neural plate mediolateral pattern formation; transcription from RNA polymerase II promoter; developmental growth; hindlimb morphogenesis; neural crest cell development; positive regulation of transcription, DNA-dependent; paraxial mesoderm structural organization; mesendoderm development; dorsal/ventral axis specification; palate development; protein amino acid phosphorylation; negative regulation of neurogenesis; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; ectoderm development; Mullerian duct regression; somitogenesis; in utero embryonic development; lateral mesoderm development; stem cell maintenance; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; mesoderm formation; pituitary gland development; cartilage development; embryonic organ development; immune response; embryonic digit morphogenesis; positive regulation of epithelial cell proliferation; regulation of lateral mesodermal cell fate specification; lung development

Disease: Juvenile Polyposis Syndrome; Polyposis Syndrome, Hereditary Mixed, 2