Goat CXCR4 Polyclonal Antibody | anti-CXCR4 antibody

Goat Polyclonal to Mouse CXCR4

Cat. Num. AAA242498

• Gene Names: CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
• Reactivity: Human, Mouse; Reactivity: Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CXCR4; Polyclonal Antibody; Goat Polyclonal to Mouse CXCR4; Anti-CXCR4 Antibody (aa14-40) IHC-plus; CD184; CD184 antigen; Cmkar4; Cxc chemokine receptor 4; CXCR-4; CXC-R4; D2S201E; FB22; Fusin; HM89; LCR1; NPY3R; NPYRL; NPYY3R; Npyy3; HSY3RR; SDF-1 receptor; WHIM; C-x-c chemokine receptor 4; LESTR; Neuropeptide Y receptor Y3; NPYR; Mouse CXCR4; anti-CXCR4 antibody

• Host: Goat
• Reactivity: Human, Mouse; Reactivity: Rat
• Clonality: Polyclonal
• Specificity: This antibody binds to CXCR4 receptor on mouse spleen leukocytes as determined by immunocytochemistry.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10mM KHPO4, 140 mM NaCI, 1mg/mL BSA, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 352

• Applicable Applications for anti-CXCR4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (5 ug/ml); ELISA (1:25000); WB (1:500); ; Optimal dilution to be determined by the researcher.
• Target: CXCR4
• Family/Subfamily: GPCR/Chemokine.
• Immunogen Species: CXCR4 antibody was raised against Mouse.
• Immunogen: CXCR4 antibody was raised against ySEEVGSGDYDSNKEPCFRDENVHFNR corresponding to the N-terminal extracellular domain of mouse CXCR4 receptor. Percent identity by BLAST analysis: Mouse (100%); Rat (93%); Baboon, Monkey, Marmoset, Elephant, Rabbit (81%).
• Epitope: aa14-40
• Usage summary: Immunohistochemistry: MBS242498 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alakaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specifity. The optimal working concentration for MBS242498 was determined to be 5 ug/mL.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CXCR4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CXCR4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CXCR4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-CXCR4 antibody

CXCR4, a Chemokine Receptor involved in organ vascularization, neuronal cell migration, and patterning of the central nervous system during development. It binds stromal cell-derived factor 1 (SDF1, also called PBFS) and mediates migration of resting leukocytes and is unique in homing hematopoietic progenitors to bone marrow. These properties suggest that CXCR4 is involved in tumor cell migration and local tumor invasion.

Product Categories/Family for anti-CXCR4 antibody

Family: GPCR; Subfamily: Chemokine

NCBI and Uniprot Product Information

• NCBI GI #: 4503175
• NCBI GeneID: 7852
• NCBI Accession #: NP_003458.1
• NCBI GenBank Nucleotide #: NM_003467.2
• UniProt Accession #: P61073; O60835; P30991; P56438; Q53S69; Q9BXA0; Q9UKN2; B2R5N0
• NCBI Official Full Name: C-X-C chemokine receptor type 4 isoform b
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 4
• NCBI Official Symbol: CXCR4
• NCBI Official Synonym Symbols: FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
• NCBI Protein Information: C-X-C chemokine receptor type 4; fusin; CD184 antigen; SDF-1 receptor; LPS-associated protein 3; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-segment receptor, spleen; leukocyte-derived seven transmembrane domain receptor
• UniProt Protein Name: C-X-C chemokine receptor type 4
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR4
• UniProt Synonym Gene Names: CXC-R4; CXCR-4; LESTR; LAP-3; LPS-associated protein 3; SDF-1 receptor
• UniProt Entry Name: CXCR4_HUMAN

NCBI Description

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CXCR4: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Monomer. Can form dimers. Interacts with CD164. Interacts with HIV-1 surface protein gp120 and Tat. Interacts with ARRB2; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and allows activation of MAPK1 and MAPK3. Interacts with ARRC; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and modulates calcium mobilization. Interacts (via the cytoplasmic C-terminal) with ITCH (via the WW domains I and II); the interaction, enhanced by CXCL12, ubiquitinates CXCR4 and leads to its degradation. Interacts with extracellular ubiquitin. Interacts with human cytomegalovirus/HHV- 5 protein UL78. Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: cell surface; lysosome; early endosome; leading edge; cytoplasmic membrane-bound vesicle; integral to membrane; intercellular junction; growth cone; cytoplasm; late endosome; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: viral receptor activity; G-protein coupled receptor activity; ubiquitin binding; protein binding; ubiquitin protein ligase binding; cytokine binding; coreceptor activity; myosin light chain binding; actin binding; C-X-C chemokine receptor activity

Biological Process: entry of virus into host cell; regulation of chemotaxis; viral reproduction; apoptosis; activation of MAPK activity; neuron migration; motor axon guidance; regulation of cell migration; germ cell development; T cell proliferation; elevation of cytosolic calcium ion concentration; germ cell migration; dendritic cell chemotaxis; positive regulation of oligodendrocyte differentiation; inflammatory response; neutrophil activation; response to virus; calcium-mediated signaling; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; ameboidal cell migration; myelin maintenance; response to hypoxia; entry into host cell; brain development

Disease: Whim Syndrome