Rabbit OXA1L Polyclonal Antibody | anti-OXA1L antibody

OXA1L Antibody

Cat. Num. AAA9603665

• Gene Names: OXA1L; OXA1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: OXA1L; Polyclonal Antibody; OXA1L Antibody; HSA; Mitochondrial inner membrane protein OXA1L; OXA1; OXA1 like protein; OXA1-like protein; OXA1Hs; Oxa1l; OXA1L_HUMAN; Oxidase (cytochrome c) assembly 1 like; Oxidase assembly 1 like protein; Oxidase assembly 1-like protein; anti-OXA1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OXA1L antibody detects endogenous levels of OXA1L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 495

• Applicable Applications for anti-OXA1L antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human OXA1L.
• Subcellular Location: Mitochondrion Inner Membrane.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of NIH/3T3 cells, using OXA1L antibody.)

Immunohistochemistry (IHC)

(MBS9603665 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-OXA1L antibody

Function: Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase. Required for the correct biogenesis of ATP synthase and complex I in mitochondria.
Subunit Structure: Monomer; predominantly monomeric at low salt concentrations. Homooligomer; predominantly homooligomeric at high salt concentrations. Homodimer. Homotetramer. Interacts with MRPL13, MRPL20, MRPL28, MRPL48, MRPL49 and MRPL51. Associates preferentially as a dimer with the large ribosomal subunit 39S of the mitochondrial ribosome.
Similarity: Belongs to the OXA1/ALB3/YidC family.

NCBI and Uniprot Product Information

• NCBI GI #: 223718097
• NCBI GeneID: 5018
• NCBI Accession #: NP_005006.3
• NCBI GenBank Nucleotide #: NM_005015.3
• UniProt Accession #: Q15070; B4DPA2
• Molecular Weight: Observed: 42 kDa; Predicted: 49 kDa
• NCBI Official Full Name: mitochondrial inner membrane protein OXA1L
• NCBI Official Synonym Full Names: OXA1L, mitochondrial inner membrane protein
• NCBI Official Symbol: OXA1L
• NCBI Official Synonym Symbols: OXA1
• NCBI Protein Information: mitochondrial inner membrane protein OXA1L
• UniProt Protein Name: Mitochondrial inner membrane protein OXA1L
• Protein Family: Mitochondrial inner membrane protein
• UniProt Gene Name: OXA1L
• UniProt Synonym Gene Names: OXA1-like protein

NCBI Description

This gene encodes an evolutionarily conserved protein that is localized to the inner mitochondrial membrane. The encoded protein is essential for the translocation of the N-terminal tail of subunit 2 of cytochrome c oxidase, and is involved in the assembly of the cytochrome c oxidase and ATPase complexes of the mitochondrial respiratory chain. [provided by RefSeq, Jul 2016]

Uniprot Description

Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase. Required for the correct biogenesis of ATP synthase and complex I in mitochondria.