Rabbit anti-Human OXA1L Polyclonal Antibody | anti-OXA1L antibody

OXA1L, CT (OXA1L, Mitochondrial inner membrane protein OXA1L, Hsa, OXA1Hs, Oxidase assembly 1-like protein) (PE)

Cat. Num. AAA6330009

• Gene Names: OXA1L; OXA1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OXA1L; Polyclonal Antibody; CT (OXA1L; Mitochondrial inner membrane protein OXA1L; Hsa; OXA1Hs; Oxidase assembly 1-like protein) (PE); mitochondrial inner membrane protein OXA1L; Oxidase assembly 1-like protein; anti-OXA1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-OXA1L antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: OXA1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-400 amino acids from the C-terminal region of human OXA1L.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(OXA1L Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the OXA1L antibody detected the OXA1L protein (arrow).)

Flow Cytometry (FC/FACS)

(OXA1L Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-OXA1L antibody

OXA1L is required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase.

Product Categories/Family for anti-OXA1L antibody

Antibodies; Enzymes; Oxidase

References

Haque, M.E., et al. J. Biol. Chem. 285(45):34991-34998(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Haque, M.E., et al. J. Biol. Chem. 285(36):28353-28362(2010). Sato, T., et al. J. Biol. Chem. 284(22):14819-14827(2009). Stiburek, L., et al. J. Mol. Biol. 374(2):506-516(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 223718097
• NCBI GeneID: 5018
• NCBI Accession #: NP_005006.3
• NCBI GenBank Nucleotide #: NM_005015.3
• UniProt Accession #: Q15070; B4DPA2
• Molecular Weight: Calculated: 49kDa; Observed: 42kDa
• NCBI Official Full Name: mitochondrial inner membrane protein OXA1L
• NCBI Official Synonym Full Names: OXA1L, mitochondrial inner membrane protein
• NCBI Official Symbol: OXA1L
• NCBI Official Synonym Symbols: OXA1
• NCBI Protein Information: mitochondrial inner membrane protein OXA1L
• UniProt Protein Name: Mitochondrial inner membrane protein OXA1L
• Protein Family: Mitochondrial inner membrane protein
• UniProt Gene Name: OXA1L
• UniProt Synonym Gene Names: OXA1-like protein
• UniProt Entry Name: OXA1L_HUMAN

NCBI Description

This gene encodes an evolutionarily conserved protein that is localized to the inner mitochondrial membrane. The encoded protein is essential for the translocation of the N-terminal tail of subunit 2 of cytochrome c oxidase, and is involved in the assembly of the cytochrome c oxidase and ATPase complexes of the mitochondrial respiratory chain. [provided by RefSeq, Jul 2016]

Uniprot Description

OXA1L: Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase. Required for the correct biogenesis of ATP synthase and complex I in mitochondria. Belongs to the OXA1/ALB3/YidC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrial membrane; mitochondrial respiratory chain; mitochondrion; protein complex

Molecular Function: protein binding; protein homodimerization activity

Biological Process: aerobic respiration; mitochondrial proton-transporting ATP synthase complex assembly; mitochondrial respiratory chain complex I assembly; negative regulation of ATPase activity; negative regulation of oxidoreductase activity; protein complex assembly; protein insertion into membrane; protein tetramerization