Rabbit ovalbumin Polyclonal Antibody
Rabbit anti Chicken ovalbumin
Reactivity
Inter-species cross reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Applications
Precipitation assays
Synonyms
ovalbumin; Polyclonal Antibody; Rabbit anti Chicken ovalbumin; anti-ovalbumin antibody
Host
Rabbit
Reactivity
Inter-species cross reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Clonality
Polyclonal
Specificity
A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilution of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum.
Form/Format
Delipidate, heat, inactivated, lyophilized,stable whole serum.
Applicable Applications for anti-ovalbumin antibody
Precipitation assays
Application Notes
Precipitin titre not less than 1:16 when tested against an 1% solution of chicken egg albymin in agar-block immunodifussion titration.
Preservative
No preservative added.
Immunogen
Highly purified egg albumin (MW 45,000)isolated from chicken egg whites.
Freund's complete adjuvants is used in the first step of the immunization procedure.
Freund's complete adjuvants is used in the first step of the immunization procedure.
Protein Concentration
Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum.No foreign proteins added.
Intended use
In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion( Mancini, Ouchterlony) to identify the presence of chicken ovalbumin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
Preparation and Storage
Lyophilized at 4°C: at least 10 years.
Reconstituted at or below -20°C: 3-5 years.
Reconstituted at 4°C: 7 days.
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Reconstituted at or below -20°C: 3-5 years.
Reconstituted at 4°C: 7 days.
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Product Categories/Family for anti-ovalbumin antibody
NCBI and Uniprot Product Information
Uniprot Description
ovalbumin: Non-inhibitory serpin. Storage protein of egg white. Belongs to the serpin family. Ov-serpin subfamily.
Cellular Component: extracellular space; extracellular region; phagocytic vesicle; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding
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Product Notes
The ovalbumin (Catalog #AAA573076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Chicken ovalbumin reacts with Inter-species cross reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. and may cross-react with other species as described in the data sheet. AAA Biotech's ovalbumin can be used in a range of immunoassay formats including, but not limited to, Precipitation assays. Precipitin titre not less than 1:16 when tested against an 1% solution of chicken egg albymin in agar-block immunodifussion titration. Researchers should empirically determine the suitability of the ovalbumin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ovalbumin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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