Rabbit anti-Human Histone H3 Monoclonal Antibody | anti-HIST3H3 antibody

Histone H3, dimethyl (Arg2) (Biotin)

Cat. Num. AAA6242929

• Gene Names: HIST3H3; H3t; H3.4; H3/g; H3FT
• Reactivity: Human
• Applications: Dot Blot, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Histone H3; Monoclonal Antibody; dimethyl (Arg2) (Biotin); anti-HIST3H3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 4H342
• Specificity: Recognizes dimethyl-arginine at residue 2 of histone H3, Mr 17kD. Species Crossreactivity: Human. Broad species crossreactivity is expected.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HIST3H3 antibody: Dot Blot (DB), Western Blot (WB)
• Immunogen: KLH-conjugated, synthetic peptide containing the sequence ...Ame2RTKQ..., in which me2R corresponds to dimethyl-arginine 2 of human histone H3.
• Conjugate: Biotin
• Preparation and Storage: Store at -20 degree C.

Testing Data

(IB: Acid extracts from HeLa cells (17-306, Lane 1) or recombinant Histone H3 (14-411, Lane 2) were probed with MBS603561 Histone H3 (Arg2) (1:6,000 dilution). )

Product Categories/Family for anti-HIST3H3 antibody

Antibodies; Histones

References

Bannister, A.J., et al., Cell 109: 801-806, 2002. General References: 1. Spieker-Polet H, et al. Proc Natl Acad Sci. 1995 Sep 26;92(20):9348-52. 2. Liguori MJ, et al. Hybridoma. 2001 Jun; 20(3):189-98. 3. G.Cano1, F. Milanezi2, D. Leitao2,3, S. Ricardo2, M.J. Brito1, F. C. Schmitt2-3 1Garcia da Orta Hospital, Almada, Portugal,2 Inst. Molec. Pathology and Immunology of Porto University, Portugal,3 Medical Faculty of Porto university, Portugal Diagn Cytopathol, 2003 Oct; 29(4): 207 -11. 4. L.K. Diaz* and N.Sneige *Department of Pathology,Northwestern University, Chicago,+ Department of Pathology, University of Texas, Huston, Adv Anat Pathol,2005; 12(1), 10-19. 5. Z. Huang1, W. Zhu2, G. Szekeres3, H. Xia1 1Spring Bioscience Corp, Fremont,CA, 2 Epitomics Inc, Burlingame,CA,, 4Histopathology Ltd, Hungary, Appl Immunohistochem Mol Morphol. 2005; 13 (1): 91-95 6. S. Rossi1, E. Orvieto1, S.Chinellato1, A. Furlanetto1, L.Laurino1, F. Facchetti2, AP Dei Tos 2 1Department of Pathology, 2Treviso, Italy; *Brescia, University School of Medicine, Brescia, Italy., Abstract presented at USCAP 2004. Modern Pathology 2004; 17 (suppl 1): 361A 7. M. Blechner, E. Ballesteros, D. Mandich, D. Stevens, R. Cartun, Hartford Hospital, Hartford, CT. Abstract presented at USCAP 2004. Modern Pathology 2004; 17 (suppl 1): 241A 8. W. Cheuk, K.O.Y. Wong, C.S.C. Wong and J.K.C. Chan, Department of Pathology, Queen Elizabeth Hospital, Hong Kong, Am J Surg Path, 2004; 28 (6): 801-807. 9. G.B. Budd, E. Tso, B. Yoder, T. Choueiri, P. Elson, S. Tarr, M. Skacel, R. Tubbs, A. Dawson, D. Hicks, Cleveland Clinic Foundation, Cleveland, OH, Abstract presented at ASCO Annual meeting, June 2004, New Orleans 10. S. M. Tarr, S. Short, K. Hansen, T. Morken, H. Xia, E. Downs-Kelly, R. R. Tubbs, D. G. Hicks, Department of Pathology and Laboratory Medicine. The Cleveland Clinic Foundation, Cleveland, Ohio. Lab Vision Corp., Fremont, Ca., Spring Bioscience Corp, Fremont,CA, Abstract presented at Association for Molecular Pathology meeting, Los Angeles, 2004 11. A.M. Gown, T.S. Barry, P. Kandalaft, L.C. Goldstein, C.C. Tse and D.O. Treaba, Clinical Research Division, PhenoPath Laboratories and IMPRIS, Seattle, WA, Abstract presented at USCAP 2005. Modern Pathology 2005; 18, suppl.1,pag 35A 12. D.O. Treaba, A.W. Hing, L.C. Goldstein, T.S. Barry, P. Kandalaft, C.B. Gilks, T.O. Nielsen and A.M. Gown, Clinical Research Division, PhenoPath Laboratories and IMPRIS, Seattle, WA, USA Genetic Pathology Evaluation Centre, University of British Columbia, Vancouver, BC, Canada, Abstract presented at USCAP 2005. Modern Pathology 2005; 18, suppl.1,pag 53A 13. S. Rossi1, L. Laurino1, A. Furlanetto1, S.Chinellato1, E. Orvieto1, F. Canal1, F. Facchetti2, A.P. Dei Tos1 1 Depart. Pathology, Hospital of Treviso, Italy, 2 Brescia University School of Medicine, Brescia, Italy, Am J Clin Pathol, 2005, Aug;124(2):295-302

NCBI and Uniprot Product Information

• NCBI GI #: 4504299
• NCBI GeneID: 8290
• NCBI Accession #: NP_003484.1
• NCBI GenBank Nucleotide #: NM_003493
• UniProt Accession #: Q16695; Q6FGU4; B2R5K3
• Molecular Weight: 136
• NCBI Official Full Name: histone H3.1t
• NCBI Official Synonym Full Names: histone cluster 3, H3
• NCBI Official Symbol: HIST3H3
• NCBI Official Synonym Symbols: H3t; H3.4; H3/g; H3FT
• NCBI Protein Information: histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
• UniProt Protein Name: Histone H3.1t
• Protein Family: Histone
• UniProt Gene Name: HIST3H3
• UniProt Synonym Gene Names: H3FT; H3/t; H3t
• UniProt Entry Name: H31T_HUMAN