Rabbit anti-Human OST alpha Polyclonal Antibody | anti-OSTA antibody

OST alpha, CT (OSTA, Organic solute transporter subunit alpha) (Biotin)

Cat. Num. AAA6329759

• Gene Names: SLC51A; OSTA; OSTalpha
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: OST alpha; Polyclonal Antibody; CT (OSTA; Organic solute transporter subunit alpha) (Biotin); OSTA; Organic solute transporter subunit alpha; anti-OSTA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OST alpha.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-OSTA antibody: Flow Cytometry (FC/FACS), Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 298-327 in the C-terminal region of human OST alpha, UniProt Accession #Q86UW1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis in 293 cell line lysates (35ug/lane) using 039522. OST alpha (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using 039522 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-OSTA antibody

Antibodies; Transport Proteins

References

1. Renner, O., et al. J. Lipid Res. 49(9):2045-2054(2008). 2. Soroka, C.J., et al. BMC Cell Biol. 9, 57 (2008). 3. Sun, A.Q., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 292 (6), G1586-G1593 (2007). 4. Boyer, J.L., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 290 (6), G1124-G1130 (2006). 5. Lim, J., et al. Cell 125(4):801-814(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 217035113
• NCBI GeneID: 200931
• NCBI Accession #: NP_689885.4
• NCBI GenBank Nucleotide #: NM_152672.5
• UniProt Accession #: Q86UW1; Q6ZMC7
• Molecular Weight: 38 kDa (MW of target protein)
• NCBI Official Full Name: organic solute transporter subunit alpha
• NCBI Official Synonym Full Names: solute carrier family 51, alpha subunit
• NCBI Official Symbol: SLC51A
• NCBI Official Synonym Symbols: OSTA; OSTalpha
• NCBI Protein Information: organic solute transporter subunit alpha
• UniProt Protein Name: Organic solute transporter subunit alpha
• UniProt Gene Name: SLC51A
• UniProt Synonym Gene Names: OSTA; OST-alpha
• UniProt Entry Name: OSTA_HUMAN

Uniprot Description

OSTalpha: Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Belongs to the OST-alpha family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane; protein complex

Molecular Function: protein heterodimerization activity; protein homodimerization activity; transporter activity

Biological Process: bile acid and bile salt transport; transport