GLuD1 recombinant protein

GLuD1, 54-558aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205535

• Gene Names: GLUD1; GDH; GDH1; GLUD
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: GLuD1; 54-558aa; Human; His tag; E Coli (Denatured); Glutamate dehydrogenase 1 mitochondrial; Glutamate dehydrogenase 1; mitochondrial; GDH; GDH1; GLuD; GLuD1 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 54-558
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT

• Applicable Applications for GLuD1 recombinant protein: SDS-PAGE
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2-8 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -80 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for GLuD1 recombinant protein

Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLuD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLuD1 has a central role in nitrogen metabolism in plants and animals. GLuD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLuD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLuD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLuD1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for GLuD1 recombinant protein

Metabolism

References

Mastorodemos V., et al. (2009) Biochem Cell Biol. 87:505-516.; Tanizawa Y., et al. (2002) Diabetes. 51:712-717.;

NCBI and Uniprot Product Information

• NCBI GI #: 4885281
• NCBI GeneID: 2746
• NCBI Accession #: NP_005262
• NCBI GenBank Nucleotide #: NM_005271.4
• UniProt Accession #: P00367; Q5TBU3; B3KV55; B4DGN5
• Molecular Weight: 58.4 kDa (528aa)
• NCBI Official Full Name: glutamate dehydrogenase 1, mitochondrial isoform a
• NCBI Official Synonym Full Names: glutamate dehydrogenase 1
• NCBI Official Symbol: GLUD1
• NCBI Official Synonym Symbols: GDH; GDH1; GLUD
• NCBI Protein Information: glutamate dehydrogenase 1, mitochondrial
• UniProt Protein Name: Glutamate dehydrogenase 1, mitochondrial
• UniProt Gene Name: GLUD1
• UniProt Synonym Gene Names: GLUD; GDH 1

NCBI Description

This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016]

Uniprot Description

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate ().