Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat ORC4L Polyclonal Antibody | anti-ORC4 antibody
Goat Anti-ORC4L, Biotinylated Antibody
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Peptide ELISA: Antibody detection limit dilution 1:8000.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]
Uniprot Description
ORC4L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Defects in ORC4 are the cause of Meier-Gorlin syndrome type 2 (MGORS2). MGORS2 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. Belongs to the ORC4 family.
Chromosomal Location of Human Ortholog: 2q22-q23
Cellular Component: actin cytoskeleton; cytoplasm; nuclear chromosome, telomeric region; nuclear origin of replication recognition complex; nucleolus; nucleoplasm; nucleus; origin recognition complex
Molecular Function: DNA replication origin binding; nucleotide binding; protein binding
Biological Process: DNA replication; DNA replication initiation; G1/S transition of mitotic cell cycle
Disease: Meier-gorlin Syndrome 2
Research Articles on ORC4
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Product Notes
The ORC4 orc4 (Catalog #AAA423677) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-ORC4L, Biotinylated Antibody reacts with Human, Mouse, Rat, Dog, Cow Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ORC4L can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Chromatin Immunoprecipitation (ChIP). Western Blot: Approx 48kDa band observed in Jurkat nuclear lysates (calculated MW of 50.4kDa according to NP_002543.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: Antibody detection limit dilution 1:8000. Researchers should empirically determine the suitability of the ORC4 orc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DVRQWATSSL SWL. It is sometimes possible for the material contained within the vial of "ORC4L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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