Western Blot (WB)
(OBFC1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OBFC1 antibody detected the OBFC1 protein (arrow).)
Rabbit anti-Human OBFC1 Polyclonal Antibody | anti-Obfc1 antibody
OBFC1, CT (OBFC1, STN1, CST complex subunit STN1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, Suppressor of cdc thirteen homolog)
Gene Names
Obfc1; AAF44; AAF-44; RPA-32; AI413458; 0610009H20Rik; 2310057J23Rik
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
OBFC1; Polyclonal Antibody; CT (OBFC1; STN1; CST complex subunit STN1; Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog); Anti -OBFC1; anti-Obfc1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Obfc1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
OBFC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-306 amino acids from the C-terminal region of human OBFC1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(OBFC1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OBFC1 antibody detected the OBFC1 protein (arrow).)
Western Blot (WB)
(OBFC1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OBFC1 antibody detected the OBFC1 protein (arrow).)
Related Product Information for anti-Obfc1 antibody
OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).
Product Categories/Family for anti-Obfc1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43,486 Da
NCBI Official Full Name
Obfc1 protein
NCBI Official Synonym Full Names
oligonucleotide/oligosaccharide-binding fold containing 1
NCBI Official Symbol
Obfc1
NCBI Official Synonym Symbols
AAF44; AAF-44; RPA-32; AI413458; 0610009H20Rik; 2310057J23Rik
NCBI Protein Information
CST complex subunit STN1; alpha accessory factor 44; alpha-accessory factor of 44 kDa; suppressor of cdc thirteen homolog; replication protein A 32 kDa subunit; oligonucleotide/oligosaccharide-binding fold-containing protein 1
UniProt Protein Name
CST complex subunit STN1
Protein Family
UniProt Gene Name
Obfc1
UniProt Synonym Gene Names
Stn1; AAF-44; AAF44
UniProt Entry Name
STN1_MOUSE
Uniprot Description
OBFC1: Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Belongs to the STN1 family.
Cellular Component: nucleoplasm; chromosome, telomeric region; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromosome, telomeric region; nucleolus; chromosome; nucleus
Molecular Function: protein binding; DNA binding; nucleic acid binding; single-stranded telomeric DNA binding; single-stranded DNA binding
Biological Process: telomere maintenance; positive regulation of DNA replication
Research Articles on Obfc1
Similar Products
Product Notes
The Obfc1 obfc1 (Catalog #AAA6001023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OBFC1, CT (OBFC1, STN1, CST complex subunit STN1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, Suppressor of cdc thirteen homolog) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OBFC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Obfc1 obfc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OBFC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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