Rabbit anti-Human P4HA1 Polyclonal Antibody | anti-P4HA1 antibody

P4HA1, CT (P4HA1, P4HA, Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1) (AP)

Cat. Num. AAA6330296

• Gene Names: P4HA1; P4HA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: P4HA1; Polyclonal Antibody; CT (P4HA1; P4HA; Prolyl 4-hydroxylase subunit alpha-1; Procollagen-proline; 2-oxoglutarate-4-dioxygenase subunit alpha-1) (AP); 2-oxoglutarate-4-dioxygenase subunit alpha-1; anti-P4HA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human P4HA1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 534

• Applicable Applications for anti-P4HA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 414-442 from the C-terminal region of human P4HA1. Species sequence homology: bovine
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Nlot analysis in Ramos cell line lysates (35ug/lane) usingMBS6010836. This demonstrates MBS6010836 detected the P4HA1 protein (arrow).)

Related Product Information for anti-P4HA1 antibody

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Categories/Family for anti-P4HA1 antibody

Antibodies; Enzymes; Oxidase

References

1. Rose, J. Phd, et al. Mol. Med. (2010). In press: 2. Gorres, K.L., et al. PLoS ONE 4 (11), E7635 (2009). 3. Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006). 4. Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006). 5. Grimmer, C., et al. Am. J. Pathol. 169(2):491-502(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 217272849
• NCBI GeneID: 5033
• NCBI Accession #: NP_001136067.1; NP_000908.2; NP_001017962.1
• NCBI GenBank Nucleotide #: NP_001136067.1
• UniProt Accession #: P13674
• NCBI Official Full Name: prolyl 4-hydroxylase subunit alpha-1 isoform 2
• NCBI Official Synonym Full Names: prolyl 4-hydroxylase subunit alpha 1
• NCBI Official Symbol: P4HA1
• NCBI Official Synonym Symbols: P4HA
• NCBI Protein Information: prolyl 4-hydroxylase subunit alpha-1
• UniProt Protein Name: Prolyl 4-hydroxylase subunit alpha-1
• Protein Family: Prolyl 4-hydroxylase
• UniProt Gene Name: P4HA1
• UniProt Synonym Gene Names: P4HA; 4-PH alpha-1
• UniProt Entry Name: P4HA1_HUMAN

NCBI Description

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.2; Endoplasmic reticulum; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding

Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline