Western Blot (WB)
(Sample Data: Western blot analysis of bub1 in A-20 cell lysate with BUB1 antibody at (A) 1 and (B) 2 ug/ml.)
Rabbit anti-Human, Rat BUB1 Polyclonal Antibody | anti-BUB1 antibody
Anti-BUB1
Reactivity
Human, Rat
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
BUB1; Polyclonal Antibody; Anti-BUB1; anti-BUB1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the BUB1protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-BUB1 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
BUB1 antibody was raised against a 22 amino acid synthetic peptide from near the carboxy terminus of human BUB1
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of bub1 in A-20 cell lysate with BUB1 antibody at (A) 1 and (B) 2 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of bub1 in A-20 cell lysate with BUB1 antibody at (A) 1 and (B) 2 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of BUB1 in A20 cells with Bub1 antibody at 5 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of BUB1 in A20 cells with Bub1 antibody at 5 ug/ml.)
Related Product Information for anti-BUB1 antibody
BUB1 is a kinase which involved in spindle checkpoint function that in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. BUB1 is highly expreesed in thymus, ovary, and testis (1). BUB1 maintains the steady-state level and centromeric localization of SGO1, and they concluded that BUB1 protects centromeric cohesion in mitosis through SGO1 (2). BUB1-mediated phosphorylation of CDC20 is required for proper checkpoint signaling and that inhibition of APC by BUB1 may partly account for the sensitivity of the spindle checkpoint. BUB1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes.
Product Categories/Family for anti-BUB1 antibody
References
1. Leland, S.et.al: Heterozygosity for a Bub1 mutation causes female-specific germ cell aneuploidy in mice. Proc. Nat. Acad. Sci. 106: 12776-12781, 2009.
2. Tang, Z.et.al: Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis. Proc. Nat. Acad. Sci. 101: 18012-18017, 2004.
2. Tang, Z.et.al: Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis. Proc. Nat. Acad. Sci. 101: 18012-18017, 2004.
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Product Notes
The BUB1 (Catalog #AAA515865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BUB1 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BUB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the BUB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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