Rabbit anti-Human OBFC1 Polyclonal Antibody | anti-OBFC1 antibody

OBFC1, CT (OBFC1, STN1, CST complex subunit STN1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, Suppressor of cdc thirteen homolog) (FITC)

Cat. Num. AAA6327494

• Gene Names: OBFC1; STN1; AAF44; AAF-44; RPA-32; bA541N10.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OBFC1; Polyclonal Antibody; CT (OBFC1; STN1; CST complex subunit STN1; Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog) (FITC); Suppressor of cdc thirteen homolog; anti-OBFC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-OBFC1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: OBFC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-306 amino acids from the C-terminal region of human OBFC1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OBFC1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OBFC1 antibody detected the OBFC1 protein (arrow).)

Related Product Information for anti-OBFC1 antibody

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).

Product Categories/Family for anti-OBFC1 antibody

Antibodies; Binding Proteins

References

Levy, D., et al. Proc. Natl. Acad. Sci. U.S.A. 107(20):9293-9298(2010). Miyake, Y., et al. Mol. Cell 36(2):193-206(2009). Wan, M., et al. J. Biol. Chem. 284(39):26725-26731(2009). Casteel, D.E., et al. J. Biol. Chem. 284(9):5807-5818(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 194394165
• NCBI GeneID: 79991
• NCBI Accession #: NP_079204.2
• NCBI GenBank Nucleotide #: NM_024928.4
• UniProt Accession #: Q9H668; Q5TCZ0; D3DR99
• Molecular Weight: 42,119 Da
• NCBI Official Full Name: CST complex subunit STN1
• NCBI Official Synonym Full Names: oligonucleotide/oligosaccharide-binding fold containing 1
• NCBI Official Symbol: OBFC1
• NCBI Official Synonym Symbols: STN1; AAF44; AAF-44; RPA-32; bA541N10.2
• NCBI Protein Information: CST complex subunit STN1; alpha accessory factor 44; oligonucleotide/oligosaccharide-binding fold-containing protein 1; replication protein A 32 kDa subunit; suppressor of cdc thirteen homolog
• UniProt Protein Name: CST complex subunit STN1
• Protein Family: CST complex
• UniProt Gene Name: OBFC1
• UniProt Synonym Gene Names: STN1
• UniProt Entry Name: STN1_HUMAN

NCBI Description

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]

Uniprot Description

OBFC1: Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Belongs to the STN1 family.

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromosome, telomeric region; nucleolus; nucleus

Molecular Function: protein binding; single-stranded telomeric DNA binding; single-stranded DNA binding

Biological Process: telomere maintenance; positive regulation of DNA replication