Rabbit anti-Human OBFC1 Polyclonal Antibody | anti-OBFC1 antibody

OBFC1, CT (OBFC1, STN1, CST complex subunit STN1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, Suppressor of cdc thirteen homolog) (Azide free) (HRP)

Cat. Num. AAA6327495

• Gene Names: OBFC1; STN1; AAF44; AAF-44; RPA-32; bA541N10.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OBFC1; Polyclonal Antibody; CT (OBFC1; STN1; CST complex subunit STN1; Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog) (Azide free) (HRP); Suppressor of cdc thirteen homolog; anti-OBFC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-OBFC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: OBFC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-306 amino acids from the C-terminal region of human OBFC1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OBFC1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OBFC1 antibody detected the OBFC1 protein (arrow).)

Related Product Information for anti-OBFC1 antibody

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).

Product Categories/Family for anti-OBFC1 antibody

Antibodies; Binding Proteins

References

Levy, D., et al. Proc. Natl. Acad. Sci. U.S.A. 107(20):9293-9298(2010). Miyake, Y., et al. Mol. Cell 36(2):193-206(2009). Wan, M., et al. J. Biol. Chem. 284(39):26725-26731(2009). Casteel, D.E., et al. J. Biol. Chem. 284(9):5807-5818(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 194394165
• NCBI GeneID: 79991
• NCBI Accession #: NP_079204.2
• NCBI GenBank Nucleotide #: NM_024928.4
• UniProt Accession #: Q9H668; Q5TCZ0; D3DR99
• Molecular Weight: 42,119 Da
• NCBI Official Full Name: CST complex subunit STN1
• NCBI Official Synonym Full Names: oligonucleotide/oligosaccharide-binding fold containing 1
• NCBI Official Symbol: OBFC1
• NCBI Official Synonym Symbols: STN1; AAF44; AAF-44; RPA-32; bA541N10.2
• NCBI Protein Information: CST complex subunit STN1; alpha accessory factor 44; oligonucleotide/oligosaccharide-binding fold-containing protein 1; replication protein A 32 kDa subunit; suppressor of cdc thirteen homolog
• UniProt Protein Name: CST complex subunit STN1
• Protein Family: CST complex
• UniProt Gene Name: OBFC1
• UniProt Synonym Gene Names: STN1
• UniProt Entry Name: STN1_HUMAN

NCBI Description

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]

Uniprot Description

OBFC1: Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Belongs to the STN1 family.

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromosome, telomeric region; nucleolus; nucleus

Molecular Function: protein binding; single-stranded telomeric DNA binding; single-stranded DNA binding

Biological Process: telomere maintenance; positive regulation of DNA replication