Western Blot (WB)
(Western blot analysis of extracts from HepG2, Hela, CHO-K1 and CHO-K1 transfected pcDNA3.1-HA-PLAP cell lysates using Placental alkaline phosphatase (PLAP) mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
anti-Human Placental alkaline phosphatase (PLAP) Monoclonal Antibody | anti-PLAP antibody
Anti-Placental alkaline phosphatase (PLAP) Mouse mAb
Gene Names
ALPP; ALP; PALP; PLAP; PLAP-1
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Placental alkaline phosphatase (PLAP); Monoclonal Antibody; Anti-Placental alkaline phosphatase (PLAP) Mouse mAb; Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; placental type; PLAP; PLAP-1; PLAP1; PPB1_HUMAN; anti-PLAP antibody
Host
Mouse
Source: Human
Source: Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8A11-C11-C9
Purity/Purification
Affinity Purified
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Sequence Length
535
Applicable Applications for anti-PLAP antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
Purified recombinant Placental alkaline phosphatase (PLAP) protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of Placental alkaline phosphatase (PLAP) and does not cross-react with related proteins.
Sequence Similarities
Belongs to the alkaline phosphatase family.
Subcellular Location
Cell membrane, membrane
Antibody type
Primary antibody
Conjugation
Unconjugation
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of extracts from HepG2, Hela, CHO-K1 and CHO-K1 transfected pcDNA3.1-HA-PLAP cell lysates using Placental alkaline phosphatase (PLAP) mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, Hela, CHO-K1 and CHO-K1 transfected pcDNA3.1-HA-PLAP cell lysates using Placental alkaline phosphatase (PLAP) mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Related Product Information for anti-PLAP antibody
Swiss-Prot Acc.P05187. Mammalian alkaline phosphatases (APs) are highly conserved zinc-containing allosteric enzymes that are able to hydrolyze and transnhosphcrylare a wide range of compounds. There are four known human alkaline phosphatase isozymes: TNAP (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPP2 (germ cell) and ALPI (intestinal). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor. It is expressed primarily In the placenta ana may be involved In trarsplacental lgG transport. ALPP has been found to be overexpressed on tne surface of several different types of solid tumor cells and elevated serum concentrations of ALPP and ALPP-Iike enzymes has been found to be associated with ovanan, cervical, and testicular cancer.
Product Categories/Family for anti-PLAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kDa
NCBI Official Full Name
alkaline phosphatase, placental type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental
NCBI Official Symbol
ALPP
NCBI Official Synonym Symbols
ALP; PALP; PLAP; PLAP-1
NCBI Protein Information
alkaline phosphatase, placental type
UniProt Protein Name
Alkaline phosphatase, placental type
Protein Family
UniProt Gene Name
ALPP
UniProt Synonym Gene Names
PLAP; PLAP-1
NCBI Description
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
Research Articles on PLAP
Similar Products
Product Notes
The PLAP alpp (Catalog #AAA475496) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Placental alkaline phosphatase (PLAP) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Placental alkaline phosphatase (PLAP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the PLAP alpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Placental alkaline phosphatase (PLAP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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