Western Blot (WB)
(Western blot analysis of NUB1 expression in Jurkat (A); Raji (B); HepG2 (C) whole cell lysates.)
Rabbit NUB1 Polyclonal Antibody | anti-NUB1 antibody
NUB1 Antibody
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Chromatography
Synonyms
NUB1; Polyclonal Antibody; NUB1 Antibody; Purified Polyclonal NUB1 Antibody; Polyclonal NUB1 antibody; Anti-NUB1 antibody; NYREN18 antibody; NEDD8 ultimate buster 1 antibody; Negative regulator of ubiquitin-like proteins 1 antibody; Renal carcinoma antigen NY-REN-18 antibody; anti-NUB1 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of NUB1 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
639
Applicable Applications for anti-NUB1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IHC: 1:100-1:200
Immunogen
NUB1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NUB1 as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of NUB1 expression in Jurkat (A); Raji (B); HepG2 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NUB1 expression in Jurkat (A); Raji (B); HepG2 (C) whole cell lysates.)
Related Product Information for anti-NUB1 antibody
NUB1 is a specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Product Categories/Family for anti-NUB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
NEDD8 ultimate buster 1 isoform 1
UniProt Protein Name
NEDD8 ultimate buster 1
Protein Family
UniProt Gene Name
NUB1
UniProt Synonym Gene Names
NYREN18
UniProt Entry Name
NUB1_HUMAN
Uniprot Description
NYREN18: Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: protein binding
Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination
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Product Notes
The NUB1 nub1 (Catalog #AAA5315616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NUB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:100-1:200. Researchers should empirically determine the suitability of the NUB1 nub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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