Rabbit RCAN1/Dscr1 Polyclonal Antibody | anti-RCAN1 antibody

RCAN1/Dscr1 (a.a. 132-140) Antibody

Cat. Num. AAA474554

• Gene Names: RCAN1; CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: RCAN1/Dscr1; Polyclonal Antibody; RCAN1/Dscr1 (a.a. 132-140) Antibody; RCAN1/Dscr1 (a.a. 132-140); anti-RCAN1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of mouse C2C12, rat PC12, and human Jurkat cells. The predominate isoform detected is the 38kDa RCAN1L, but the antibody may also detect RCAN2 and RCAN3.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-RCAN1 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:300; WB: 1:1000
• Immunogen: RCAN1 synthetic peptide corresponding to amino acids 132 to 140 in human RCAN1S (isoform 2). This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1. In addition, this sequence has significant homology to RCAN2 and RCAN3.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of RCAN1 expression in human Jurkat (lanes 1 & 4), mouse C2C12 (lanes 2 & 5), and rat PC12 (lanes 3 & 6). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000 and anti-RCAN1 (a.a. 132-140) at 1:500.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (RP3941) and anti-RCAN1 (a.a. 132-140) (RP3961) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.)

Related Product Information for anti-RCAN1 antibody

An important element of calcium signaling pathways involves calmodulin activation of calcineurin (phosphatase PP2B), leading to dephosphorylation of transcription factors such as NFAT and MEF2. A wide variety of proteins other than calmodulin have also been implicated in regulating calcineurin activity. Regulators of Calcineurin (RCANs) include RCAN1, RCAN2, and RCAN3. RCAN1 has previously been referred to as Down's syndrome candidate region-1 (Dscr1), MCIP, calcipressin, and Adapt78. This RCAN is expressed as several different variants with RCAN1L (38kDa) and RCAN1S (31kDa) being most prevalent. RCAN1 is increased in Down's syndrome tissues and in a mouse model of Down's syndrome. Increased expression of RCAN1 leads to significant suppression of tumor growth in mice as result of deficits in calcineurin-induced tumor angiogenesis. RCAN1 can recruit TAB1, TAK1, and calcineurin into a macromolecular signaling complex, and TAK1 can phosphorylate Ser-94 and Ser-136 in RCAN1S. This phosphorylation converts RCAN1 from an inhibitor to a facilitator of calcineurin-NFAT signaling.

References

Baek, K.H. et al. (2009) Nature. 459(7250):1126.
Liu, Q. et al. (2009) Nat Cell Biol. 11:154.
Genesca, L. et al. (2003) Biochem J. 374:567.

NCBI and Uniprot Product Information

• NCBI GI #: 33620725
• NCBI GeneID: 1827
• NCBI Accession #: NP_004405.3
• NCBI GenBank Nucleotide #: NM_004414.5
• UniProt Accession #: P53805; O00582; O00583; Q53XT0; Q6IBC6; Q7Z555; Q96R03; Q9BU69; Q9UF15; Q9UME4; D3DSF9
• Molecular Weight: 28,079 Da
• NCBI Official Full Name: calcipressin-1 isoform a
• NCBI Official Synonym Full Names: regulator of calcineurin 1
• NCBI Official Symbol: RCAN1
• NCBI Official Synonym Symbols: CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
• NCBI Protein Information: calcipressin-1; near DSCR proline-rich protein; Down syndrome candidate region 1; calcium and oxidant-inducible mRNA; Down syndrome critical region gene 1; down syndrome critical region protein 1; modulatory calcineurin-interacting protein 1; myocyte-enri
• UniProt Protein Name: Calcipressin-1
• Protein Family: Calcipressin
• UniProt Gene Name: RCAN1
• UniProt Synonym Gene Names: ADAPT78; CSP1; DSC1; DSCR1; MCIP1
• UniProt Entry Name: RCAN1_HUMAN

NCBI Description

The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RCAN1: Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development. Interacts with RAF1. By calcium. Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues. Belongs to the RCAN family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 21q22.12

Cellular Component: nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; transcription factor activity

Biological Process: central nervous system development; regulation of transcription, DNA-dependent; blood circulation; calcium-mediated signaling; signal transduction