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Western Blot (WB) (Western blotAll lanes: MTHFS antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 24,21 kDaObserved band size: 23 kDa)

Rabbit anti-Human MTHFS Polyclonal Antibody | anti-MTHFS antibody

MTHFS Antibody

Gene Names
MTHFS; HsT19268
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
MTHFS; Polyclonal Antibody; MTHFS Antibody; 5-formyltetrahydrofolate cyclo-ligase; 5; 10-methenyl-tetrahydrofolate synthetase; Methenyl-THF synthetase; anti-MTHFS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
146
Applicable Applications for anti-MTHFS antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human 5-formyltetrahydrofolate cyclo-ligase protrin (2-203AA)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-66469
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: MTHFS antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 24,21 kDaObserved band size: 23 kDa)

Western Blot (WB) (Western blotAll lanes: MTHFS antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 24,21 kDaObserved band size: 23 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung tissue using MBS7005112 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung tissue using MBS7005112 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of MCF-7 cells using MBS7005112 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of MCF-7 cells using MBS7005112 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-MTHFS antibody
Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,519 Da
NCBI Official Full Name
5-formyltetrahydrofolate cyclo-ligase isoform b
NCBI Official Synonym Full Names
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
NCBI Official Symbol
MTHFS
NCBI Official Synonym Symbols
HsT19268
NCBI Protein Information
5-formyltetrahydrofolate cyclo-ligase
UniProt Protein Name
5-formyltetrahydrofolate cyclo-ligase
UniProt Gene Name
MTHFS
UniProt Synonym Gene Names
MTHFS; Methenyl-THF synthetase
UniProt Entry Name
MTHFS_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.

Research Articles on MTHFS

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Product Notes

The MTHFS mthfs (Catalog #AAA7005112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTHFS Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MTHFS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the MTHFS mthfs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTHFS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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