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Western Blot (WB) (Western Blot analysis of CD81 on different lysates using anti-CD81 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR Lane 2: PC-12 Lane 3: K562)

Rabbit TAPA1/CD81 Polyclonal Antibody | anti-TAPA1 antibody

TAPA1/CD81 Polyclonal Antibody

Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
TAPA1/CD81; Polyclonal Antibody; TAPA1/CD81 Polyclonal Antibody; Tspan-28; CD81; 26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen (target of antiproliferative; 1); CD81 antigen; CD81 molecule; CVID6; S5.7; TAPA 1; TAPA1; Target of the antiproliferative; 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan28; anti-TAPA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TAPA1/CD81 polyclonal antibody detects endogenous levels of TAPA1/CD81 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
236
Applicable Applications for anti-TAPA1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of CD81 on different lysates using anti-CD81 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR Lane 2: PC-12 Lane 3: K562)

Western Blot (WB) (Western Blot analysis of CD81 on different lysates using anti-CD81 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR Lane 2: PC-12 Lane 3: K562)

Immunocytochemistry (ICC)

(ICC staining CD81 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining CD81 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-TAPA1 antibody
CD81, also called TAPA-1, is a type III transmembrane protein that is broadly expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin. CD81 is believed to be involved in both cell growth and signal transduction. It can be present as a multimolecular complex in association with CD37 and/or CD53, or on the surface of B cells in association with CD19, CD21 and/or MHC class II antigens.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
975
UniProt Accession #
Molecular Weight
20kDa
NCBI Official Full Name
CD81 antigen
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
NCBI Protein Information
CD81 antigen
UniProt Protein Name
CD81 antigen
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.

Research Articles on TAPA1

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Product Notes

The TAPA1 cd81 (Catalog #AAA3013535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAPA1/CD81 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TAPA1/CD81 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1,000-1:2,000 ICC: 1:100-1:500 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TAPA1 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAPA1/CD81, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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