Rabbit anti-Human PDE6D Polyclonal Antibody | anti-PDE6D antibody

Anti-PDE6D Antibody, Rabbit Polyclonal

Cat. Num. AAA8108639

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: PDE6D; Polyclonal Antibody; Anti-PDE6D Antibody; Rabbit Polyclonal; PDE6D Antibody; Rabbit PAb; Antigen Affinity Purified; phosphodiesterase 6D; cGMP-specific; rod; delta; Anti-JBTS22 Antibody; Anti-PDED Antibody; anti-PDE6D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PDE6D
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PDE6D antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200
• Immunogen: E Coli-derived Human PDE6D fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PDE6D fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PDE6D rabbit polyclonal antibody at 1:500 dilutionLane A: MOLT4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PDE6D in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PDE6D antibody

PrBP/delta, encoded by the Pde6d gene, is an isoprenyl-binding protein that regulates trafficking of isoprenylated proteins, such as PDE6 and GRK1, from photoreceptor inner segments to outer segments. The human gene (PDE6D) encoding a new subunit (delta) has been characterized and mapped to the long arm of chromosome 2 (HSA2q35-q36) where a new autosomal recessive retinitis pigmentosa (arRP) locus (RP26) has been localized. Characterization of the canine PDE6D shows the gene is about 4.2kb containing four exons interrupted by three introns. Overexpression of PDE6D in mouse eyes caused elevated intraocular pressure (IOP), and this elevation was reversed by topical ocular application of either anecortave acetate (AA) or anecortave desacetate (AdesA). The identification of PDE6D as the molecular binding partner of AA provides insight into the role of this drug candidate in treating glaucoma.

References

Zhang H, et al. (2014) Unc119 gene deletion partially rescues the grk1 transport defect of pde6d (-/-) cones. Adv Exp Med Biol 801 487-493.Zhang H, et al. (2007) Deletion of prbp/delta impedes transport of grk1 and pde6 catalytic subunits to photoreceptor outer segments. Proc Natl Acad Sci U S A 104 (21): 8857-8862.Wang W, et al. (1999) Molecular characterization and mapping of canine cgmp-phosphodiesterase delta subunit (pde6d). Gene 236 (2): 325-332.Shepard AR, et al. (2013) Identification of pde6d as a molecular target of anecortave acetate via a methotrexate-anchored yeast three-hybrid screen. ACS Chem Biol 8 (3): 549-558.