Immunohistochemistry (IHC)
(Paraffin-embedded colorectal carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS631605. Reactivity was detected using the IHC-Select Detection Kit Staining pattern appears to be restricted to the purkinje cells (granular layer junction).)
Mouse anti-Rat Slo1 Monoclonal Antibody
Slo1 (BKCa, BKCA alpha, BK channel, BK channel alpha subunit, Kcnma1, Potassium Large Conductance Calcium-Activated Channel, subfamily M, alpha member 1)
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Slo1; Monoclonal Antibody; Slo1 (BKCa; BKCA alpha; BK channel; BK channel alpha subunit; Kcnma1; Potassium Large Conductance Calcium-Activated Channel; subfamily M; alpha member 1); Anti -Slo1 (BKCa; anti-Slo1 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
11C233
Specificity
Recognizes S9-S10 segments of Slo1. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-Slo1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1ug/ml detects Slo1 on 10ug of rat brain membrane tissue lysate.
Immunohistochemistry: 1:500 detects Slo1 in rat cerebellum tissue.
Dilution: Western Blot: 1ug/ml detects Slo1 on 10ug of rat brain membrane tissue lysate.
Immunohistochemistry: 1:500 detects Slo1 in rat cerebellum tissue.
Immunogen
Recombinant protein corresponding to the S9-S10 segments of mouse Slo1.
Positive Control
Rat brain membrane tissue lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Paraffin-embedded colorectal carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS631605. Reactivity was detected using the IHC-Select Detection Kit Staining pattern appears to be restricted to the purkinje cells (granular layer junction).)
Immunohistochemistry (IHC)
(Paraffin-embedded colorectal carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS631605. Reactivity was detected using the IHC-Select Detection Kit Staining pattern appears to be restricted to the purkinje cells (granular layer junction).)
Testing Data
(Rat brain membrane tissue lysate was probed with MBS631605 (1ug/ml). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection system. |Arrow indicates Slo 1 (~100kD).)
Testing Data
(Rat brain membrane tissue lysate was probed with MBS631605 (1ug/ml). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection system. |Arrow indicates Slo 1 (~100kD).)
Related Product Information for anti-Slo1 antibody
Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.
Product Categories/Family for anti-Slo1 antibody
Similar Products
Product Notes
The Slo1 (Catalog #AAA631605) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Slo1 (BKCa, BKCA alpha, BK channel, BK channel alpha subunit, Kcnma1, Potassium Large Conductance Calcium-Activated Channel, subfamily M, alpha member 1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Slo1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 1ug/ml detects Slo1 on 10ug of rat brain membrane tissue lysate. Immunohistochemistry: 1:500 detects Slo1 in rat cerebellum tissue. Researchers should empirically determine the suitability of the Slo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Slo1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
