Rabbit anti-Human ANKHD1 Polyclonal Antibody | anti-ANKHD1 antibody

ANKHD1, NT (ANKHD1, KIAA1085, MASK, VBARP, Ankyrin repeat and KH domain-containing protein 1, HIV-1 Vpr-binding ankyrin repeat protein, Multiple ankyrin repeats single KH domain) (Biotin)

Cat. Num. AAA6273545

• Gene Names: ANKHD1; MASK; VBARP; PP2500
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANKHD1; Polyclonal Antibody; NT (ANKHD1; KIAA1085; MASK; VBARP; Ankyrin repeat and KH domain-containing protein 1; HIV-1 Vpr-binding ankyrin repeat protein; Multiple ankyrin repeats single KH domain) (Biotin); Multiple ankyrin repeats single KH domain; anti-ANKHD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ANKHD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ANKHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the N-terminal region of human ANKHD1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ANKHD1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ANKHD1 antibody detected the ANKHD1 protein (arrow).)

Related Product Information for anti-ANKHD1 antibody

The ANKHD1-EIF4EBP3 mRNA is an infrequent but naturally occurring readthrough transcript of the neighboring ANKHD1 and EIF4EBP3 genes. This readthrough transcript encodes a protein composed mostly of the multiple ankyrin repeats, single KH-domain protein, with its C-terminus encoded in a different reading frame from the shared portion of the EIF4EBP3 gene. The significance of this readthrough mRNA and the function of its protein product have not yet been determined.

Product Categories/Family for anti-ANKHD1 antibody

Antibodies; Infectious Disease HIV

References

Prakash, T., et al. PLoS ONE 5 (10), E13284 (2010): Santos Duarte Ada, S., et al. Gene 363, 113-122 (2005): Poulin, F., et al. J. Biol. Chem. 278(52):52290-52297(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 46519147
• NCBI GeneID: 54882
• NCBI Accession #: NP_060217.1; NP_078944.2; NP_060448.1
• NCBI GenBank Nucleotide #: NM_017747.2
• UniProt Accession #: Q8IWZ3; Q149P2; Q8IWZ2; Q8WY90; Q96G77; Q96GK0; Q9H2U0; Q9HA95; Q9NWG4; Q9UPR7; A6NH85
• Molecular Weight: 277,175 Da
• NCBI Official Full Name: ankyrin repeat and KH domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: ankyrin repeat and KH domain containing 1
• NCBI Official Symbol: ANKHD1
• NCBI Official Synonym Symbols: MASK; VBARP; PP2500
• NCBI Protein Information: ankyrin repeat and KH domain-containing protein 1; HIV-1 Vpr-binding ankyrin repeat protein; multiple ankyrin repeats, single KH-domain homolog
• UniProt Protein Name: Ankyrin repeat and KH domain-containing protein 1
• Protein Family: Ankyrin repeat and KH domain-containing protein
• UniProt Gene Name: ANKHD1
• UniProt Synonym Gene Names: KIAA1085; MASK; VBARP; hMASK
• UniProt Entry Name: ANKH1_HUMAN

NCBI Description

This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Sep 2010]