Immunofluorescence (IF)
(MBS9607088 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit NPY Polyclonal Antibody | anti-NPY antibody
NPY Antibody
Gene Names
NPY; PYY4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NPY; Polyclonal Antibody; NPY Antibody; C-flanking peptide of NPY; CPON; Neuropeptide tyrosine; Neuropeptide Y precursor; NPY_HUMAN; Pro neuropeptide Y; PYY 4; PYY4; Y Neuropeptide; anti-NPY antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NPY antibody detects endogenous levels of total NPY
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
97
Applicable Applications for anti-NPY antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:500
IHC: 1:50-1:200
IF: 1:500
Immunogen
A synthesized peptide derived from human NPY
Subcellular Location
Secreted.
Tissue Specificity
One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9607088 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9607088 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts of 293T, using NPY antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of 293T, using NPY antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9607088 at 1/200 staining Rat brain tissue sections by IHC-P.)
Immunohistochemistry (IHC)
(MBS9607088 at 1/200 staining Rat brain tissue sections by IHC-P.)
Related Product Information for anti-NPY antibody
Description: The NPY hormone family consists of NPY, PP and peptide YY. NPY (neuropeptide Y) is a 36 amino acid protein that consists of a polyproline stretch followed by an amphipathic a-helix. NPY shares a 50% amino acid homology with pancreatic polypeptide gene (PP). NPY is expressed throughout the central and peripheral nervous system, particularly in the deep layers of the cortex and smaller cell bodies in the white matter. NPY interacts with the Y-receptor family of G protein-coupled receptors. NPY interacts with NPY receptor Y1 to increase corticotropin levels and decrease noradrenaline levels in the hypothalamus. Through interactions in the hypothalus, NPY plays important roles in the regulation of energy balance by stimulating food intake. NPY favors energy storage by increasing lipoprotein lipase activity in white adipose tissue. A Leucine 7 to Proline 7 polymorphism has been implicated in an increase in alcohol preference, and decrease in cholesterol metabolism.
Function: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Post-translational Modifications: The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
Similarity: Belongs to the NPY family.
Function: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Post-translational Modifications: The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
Similarity: Belongs to the NPY family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 11 kDa
Predicted: 11 kDa
Predicted: 11 kDa
NCBI Official Full Name
pro-neuropeptide Y preproprotein
NCBI Official Synonym Full Names
neuropeptide Y
NCBI Official Symbol
NPY
NCBI Official Synonym Symbols
PYY4
NCBI Protein Information
pro-neuropeptide Y
UniProt Protein Name
Pro-neuropeptide Y
Protein Family
UniProt Gene Name
NPY
UniProt Synonym Gene Names
NPY; CPON
NCBI Description
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]
Uniprot Description
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Research Articles on NPY
Similar Products
Product Notes
The NPY npy (Catalog #AAA9607088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPY Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPY can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:500. Researchers should empirically determine the suitability of the NPY npy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
