Mouse Pro-neuropeptide Y ELISA Kit | Npy elisa kit

Mouse Pro-neuropeptide Y ELISA Kit

Cat. Num. AAA765227

• Gene Names: NPY; PYY4
• Reactivity: Mouse
• Synonyms: Pro-neuropeptide Y; Mouse Pro-neuropeptide Y ELISA Kit; Npy (Neuropeptide Y)/PYY4/170 kDa melanoma membrane-bound gelatinase/DPPIV/FAPA/Fibroblast activation protein alpha/fibroblast activation protein; alpha/Integral membrane serine protease/seprase; Npy elisa kit

• Reactivity: Mouse
• Sequence Length: 97

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Npy elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Npy antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Npy antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Npy amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Npy can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505449
• NCBI GeneID: 4852
• NCBI Accession #: NP_000896.1
• NCBI GenBank Nucleotide #: NM_000905.3
• Molecular Weight: 10,851 Da
• NCBI Official Full Name: pro-neuropeptide Y preproprotein
• NCBI Official Synonym Full Names: neuropeptide Y
• NCBI Official Symbol: NPY
• NCBI Official Synonym Symbols: PYY4
• NCBI Protein Information: pro-neuropeptide Y
• UniProt Protein Name: Pro-neuropeptide Y
• Protein Family: Pro-neuropeptide
• UniProt Gene Name: NPY
• UniProt Synonym Gene Names: NPY; CPON
• UniProt Entry Name: NPY_HUMAN

NCBI Description

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

Uniprot Description

NPY: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Belongs to the NPY family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 7p15.1

Cellular Component: cell; extracellular region; extracellular space; Golgi apparatus

Molecular Function: calcium channel regulator activity; G-protein coupled receptor activity; G-protein-coupled receptor binding; neuropeptide hormone activity; receptor binding

Biological Process: adult feeding behavior; antibacterial humoral response; antifungal humoral response; behavior; blood circulation; calcium ion transport; cell motility; cell proliferation; central nervous system neuron development; cerebral cortex development; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; digestion; feeding behavior; G-protein signaling, coupled to cyclic nucleotide second messenger; innate immune response; neurite development; neuropeptide signaling pathway; positive regulation of appetite; regulation of appetite; regulation of blood pressure; response to yeast; synaptic transmission